中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2011年
1期
33-35
,共3页
陈怡%苏东辉%潘晨%黄祖雄
陳怡%囌東輝%潘晨%黃祖雄
진이%소동휘%반신%황조웅
肝炎,乙型,慢性%儿童%干扰素γ%白细胞介素4%流式细胞技术
肝炎,乙型,慢性%兒童%榦擾素γ%白細胞介素4%流式細胞技術
간염,을형,만성%인동%간우소γ%백세포개소4%류식세포기술
Chronic Hepatitis B%Child%Interferon γ%Interleukin-4%Flow cytometry
目的 检测细胞内外IFNγ和IL-4水平,评估慢性乙型肝炎(CHB)和HBV携带患儿Th1/Th2应答状态.方法 收集2007年5月-2009年2月54例住院患儿,将其分为CHB组(23例)和HBV携带组(31例),另外选取34名健康儿童作为对照.采用ELISA法检测研究对象全血细胞外IFNγ和IL-4,采用流式细胞术检测细胞内IFNγ和IL-4.方差齐者行方差分析,不齐者行非参数Kruskal-Wallis检验分析.结果 ELISA法检测发现,IFNγ、IL-4水平和Th1/Th2比值在CHB组、HBV携带组和健康对照组间差异无统计学意义(F=0.342、0.020和0.507,P值均＞0.05).流式细胞术检测发现细胞内IFNγ水平在CHB组、HBV携带组和健康对照组分别为(7.68±4.62)、(11.71±4.36)和(13.61±6.71)μg/mL,Th1/Th2比值分别为0.96±0.30、1.67±0.76和2.11±1.12,差异有统计学意义(F=0.255和0.140,P＜0.05或＜0.01);IL-4水平在三组间的差异无统计学意义(F=0.425,P＞0.05).结论 CHB和HBV携带组患儿细胞内因子表达失衡,细胞免疫功能受到抑制.与ELISA法相比,流式细胞技术能够更加真实、准确地反映Th1和Th2的应答状态.
目的 檢測細胞內外IFNγ和IL-4水平,評估慢性乙型肝炎(CHB)和HBV攜帶患兒Th1/Th2應答狀態.方法 收集2007年5月-2009年2月54例住院患兒,將其分為CHB組(23例)和HBV攜帶組(31例),另外選取34名健康兒童作為對照.採用ELISA法檢測研究對象全血細胞外IFNγ和IL-4,採用流式細胞術檢測細胞內IFNγ和IL-4.方差齊者行方差分析,不齊者行非參數Kruskal-Wallis檢驗分析.結果 ELISA法檢測髮現,IFNγ、IL-4水平和Th1/Th2比值在CHB組、HBV攜帶組和健康對照組間差異無統計學意義(F=0.342、0.020和0.507,P值均＞0.05).流式細胞術檢測髮現細胞內IFNγ水平在CHB組、HBV攜帶組和健康對照組分彆為(7.68±4.62)、(11.71±4.36)和(13.61±6.71)μg/mL,Th1/Th2比值分彆為0.96±0.30、1.67±0.76和2.11±1.12,差異有統計學意義(F=0.255和0.140,P＜0.05或＜0.01);IL-4水平在三組間的差異無統計學意義(F=0.425,P＞0.05).結論 CHB和HBV攜帶組患兒細胞內因子錶達失衡,細胞免疫功能受到抑製.與ELISA法相比,流式細胞技術能夠更加真實、準確地反映Th1和Th2的應答狀態.
목적 검측세포내외IFNγ화IL-4수평,평고만성을형간염(CHB)화HBV휴대환인Th1/Th2응답상태.방법 수집2007년5월-2009년2월54례주원환인,장기분위CHB조(23례)화HBV휴대조(31례),령외선취34명건강인동작위대조.채용ELISA법검측연구대상전혈세포외IFNγ화IL-4,채용류식세포술검측세포내IFNγ화IL-4.방차제자행방차분석,불제자행비삼수Kruskal-Wallis검험분석.결과 ELISA법검측발현,IFNγ、IL-4수평화Th1/Th2비치재CHB조、HBV휴대조화건강대조조간차이무통계학의의(F=0.342、0.020화0.507,P치균＞0.05).류식세포술검측발현세포내IFNγ수평재CHB조、HBV휴대조화건강대조조분별위(7.68±4.62)、(11.71±4.36)화(13.61±6.71)μg/mL,Th1/Th2비치분별위0.96±0.30、1.67±0.76화2.11±1.12,차이유통계학의의(F=0.255화0.140,P＜0.05혹＜0.01);IL-4수평재삼조간적차이무통계학의의(F=0.425,P＞0.05).결론 CHB화HBV휴대조환인세포내인자표체실형,세포면역공능수도억제.여ELISA법상비,류식세포기술능구경가진실、준학지반영Th1화Th2적응답상태.
Objective To evaluate extracellular and intracellular IFNγ and IL-4 levels in assessing Th1/Th2 balance in children with chronic hepatitis B (CHB) and asymptomatic carriers.Methods Fiftyfour hospitalized children including 23 CHB patients and 31 asymptomatic carriers were collected from May 2007 to February 2009.Thirty-four healthy children were served as control.Serum IFNγ and IL-4 levels were measured by enzyme-linked immunosorbent assay (ELISA) and intracellular IFNγand IL-4 levels were detected by flow cytometer.Analysis of variance (for homogenous variance) and Kruskal-Wallis test (for non-homogenous variance) were performed.Results The differences on extracellular IFNγ, IL-4 and Th1/Th2 among CHB, asymptomatic carriers and control groups were not statistically significant (F=0.342, 0.020 and 0.507, P ＞ 0.05); while the intracellular IFNγlevels were (7.68 ± 4.62), (11.71 ±4.36) and (13.61 ±6.71) μg/mL, and Th1/Th2 ratios were 0.96 ±0.30, 1.67 ±0.76 and 2.11 ± 1.12in three groups respectively (F=0.255 and 0.140, P ＜ 0.05 or ＜ 0.01).The differences in intracellular IL-4 levels among three groups were not significant (F=0.425, P ＞ 0.05).Conclusions Cytokine balance is affected in CHB children and asymptomatic carriers, and flow cytometry analysis is considered as a better method in evaluating the status of Th1/Th2 balance.
