中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2010年
3期
166-169
,共4页
范义湘%石卫民%黎静%尹吉林%杨传红%黄凯龄%刘青竹%李科斌%吴继珍
範義湘%石衛民%黎靜%尹吉林%楊傳紅%黃凱齡%劉青竹%李科斌%吳繼珍
범의상%석위민%려정%윤길림%양전홍%황개령%류청죽%리과빈%오계진
鼻咽肿瘤%体层摄影术,发射型计算机%单糖转运蛋白质类%己糖激酶%脱氧葡萄糖
鼻嚥腫瘤%體層攝影術,髮射型計算機%單糖轉運蛋白質類%己糖激酶%脫氧葡萄糖
비인종류%체층섭영술,발사형계산궤%단당전운단백질류%기당격매%탈양포도당
Nasopharyngeal neoplasms%Tomography,emission-computed%Monosaccharide transport proteins%Hexokinase%Deoxyglucose
目的 测定并分析早期鼻咽癌18F-脱氧葡萄糖(FDG)摄取与肿瘤组织葡萄糖转运蛋白1(Glut1)及己糖激酶-Ⅱ(HK-Ⅱ)的表达,以探讨鼻咽癌组织摄取FDG的分子机制及其与肿瘤病理类型和分期的相关性.方法 40例早期鼻咽癌患者(T1期12例,T2期28例)行PET检查,测定病灶最大标准摄取值(SUVmax)及平均标准摄取值(SUVmean);行鼻咽部肿瘤活组织检查,并采用免疫组织化学法测定其Glut1及HK-Ⅱ表达,计算阳性细胞百分率.T1与T2期患者SUVmax或SUVmean间的差异采用t检验,Glut1及HK-Ⅱ表达阳性率差异采用x2检验,SUVmax与Glut1和HK-Ⅱ表达间的关系采用Pearson相关分析.结果 40例患者SUVmax与SUVmean分别为9.45±1.87和6.04±1.09.T1期分别为8.95±1.91和5.61±1.08,T2期分别为11.55±1.70和7.98±1.10,T1期与T2期相比原发灶SUVmax(t=4.46,P＜0.001)及SUVmean(t=6.76,P＜0.001)差异均有统计学意义.40例早期鼻咽癌患者Glut1及HK-Ⅱ染色均为阳性,阳性细胞率分别为(45.2±10.9)%与(68.3±9.5)%.其中Glut1阳性细胞率在T1期和T2期分别为(38.4±8.1)%与(49.7±12.6)%,两者差异无统计学意义(x2=40.58,P＞0.05),而HK-Ⅱ阳性细胞率在T1期与T2期分别为(60.1±11.1)%与(77.9±14.7)%,差异有统计学意义(x2=58.71,P＜0.05)).SUVmax与Glut1表达阳性细胞率呈低度正相关(r=0.369,P=0.019),与HK-Ⅱ表达阳性细胞率呈中度正相关(r=0.549,P=0.001).结论 早期鼻咽癌组织FDG摄取与Glut1过度表达相关.
目的 測定併分析早期鼻嚥癌18F-脫氧葡萄糖(FDG)攝取與腫瘤組織葡萄糖轉運蛋白1(Glut1)及己糖激酶-Ⅱ(HK-Ⅱ)的錶達,以探討鼻嚥癌組織攝取FDG的分子機製及其與腫瘤病理類型和分期的相關性.方法 40例早期鼻嚥癌患者(T1期12例,T2期28例)行PET檢查,測定病竈最大標準攝取值(SUVmax)及平均標準攝取值(SUVmean);行鼻嚥部腫瘤活組織檢查,併採用免疫組織化學法測定其Glut1及HK-Ⅱ錶達,計算暘性細胞百分率.T1與T2期患者SUVmax或SUVmean間的差異採用t檢驗,Glut1及HK-Ⅱ錶達暘性率差異採用x2檢驗,SUVmax與Glut1和HK-Ⅱ錶達間的關繫採用Pearson相關分析.結果 40例患者SUVmax與SUVmean分彆為9.45±1.87和6.04±1.09.T1期分彆為8.95±1.91和5.61±1.08,T2期分彆為11.55±1.70和7.98±1.10,T1期與T2期相比原髮竈SUVmax(t=4.46,P＜0.001)及SUVmean(t=6.76,P＜0.001)差異均有統計學意義.40例早期鼻嚥癌患者Glut1及HK-Ⅱ染色均為暘性,暘性細胞率分彆為(45.2±10.9)%與(68.3±9.5)%.其中Glut1暘性細胞率在T1期和T2期分彆為(38.4±8.1)%與(49.7±12.6)%,兩者差異無統計學意義(x2=40.58,P＞0.05),而HK-Ⅱ暘性細胞率在T1期與T2期分彆為(60.1±11.1)%與(77.9±14.7)%,差異有統計學意義(x2=58.71,P＜0.05)).SUVmax與Glut1錶達暘性細胞率呈低度正相關(r=0.369,P=0.019),與HK-Ⅱ錶達暘性細胞率呈中度正相關(r=0.549,P=0.001).結論 早期鼻嚥癌組織FDG攝取與Glut1過度錶達相關.
목적 측정병분석조기비인암18F-탈양포도당(FDG)섭취여종류조직포도당전운단백1(Glut1)급기당격매-Ⅱ(HK-Ⅱ)적표체,이탐토비인암조직섭취FDG적분자궤제급기여종류병리류형화분기적상관성.방법 40례조기비인암환자(T1기12례,T2기28례)행PET검사,측정병조최대표준섭취치(SUVmax)급평균표준섭취치(SUVmean);행비인부종류활조직검사,병채용면역조직화학법측정기Glut1급HK-Ⅱ표체,계산양성세포백분솔.T1여T2기환자SUVmax혹SUVmean간적차이채용t검험,Glut1급HK-Ⅱ표체양성솔차이채용x2검험,SUVmax여Glut1화HK-Ⅱ표체간적관계채용Pearson상관분석.결과 40례환자SUVmax여SUVmean분별위9.45±1.87화6.04±1.09.T1기분별위8.95±1.91화5.61±1.08,T2기분별위11.55±1.70화7.98±1.10,T1기여T2기상비원발조SUVmax(t=4.46,P＜0.001)급SUVmean(t=6.76,P＜0.001)차이균유통계학의의.40례조기비인암환자Glut1급HK-Ⅱ염색균위양성,양성세포솔분별위(45.2±10.9)%여(68.3±9.5)%.기중Glut1양성세포솔재T1기화T2기분별위(38.4±8.1)%여(49.7±12.6)%,량자차이무통계학의의(x2=40.58,P＞0.05),이HK-Ⅱ양성세포솔재T1기여T2기분별위(60.1±11.1)%여(77.9±14.7)%,차이유통계학의의(x2=58.71,P＜0.05)).SUVmax여Glut1표체양성세포솔정저도정상관(r=0.369,P=0.019),여HK-Ⅱ표체양성세포솔정중도정상관(r=0.549,P=0.001).결론 조기비인암조직FDG섭취여Glut1과도표체상관.
Objective To discuss the molecular mechanism of 18F-fluorodeoxyglucose (FDG) uptake in tumor and to assess its value to identify pathologic type and cancer staging in patients with earlystage nasopharyngeal carcinoma.Methods Forty patients with nasopharyngeal carcinoma of early-stage,including 12 cases with T1 stage and 28 cases with T2 stage, underwent FDG PET imaging.The maximum standardized uptake value ( SUVmax ) and mean standardized uptake value ( SUVmean ) of FDG uptake of each patient were measured and compared between T1 and T2 stage by t-test.The expression of glucose transport protein 1 ( Glut1 ) and hexokinase- Ⅱ ( HK- Ⅱ ) of each case was measured in paraffin sections by streptavidin-perosidase (SP) immunohistochemistry.The positive expression rate of Glut1 and HK- Ⅱ was calculated and compared between T1 and T2 by x2 test.Meanwhile, the correlation between the expression of Glut1 or HK-Ⅱ and the SUVmax was tested by Pearson analysis.Results The SUVmax and SUVmean in 40 patients were 9.45 ± 1.87 and 6.04 ± 1.09, respectively.The SUVmax of patients with T1 stage (8.95 ± 1.91 ) was significantly lower (t =4.46, P＜0.001 ) than that of patients with T2 stage (11.55 ± 1.70), and the SUVmean of patients with T1 stage (5.61 ± 1.08) was significantly lower ( t = 6.76, P ＜ 0.001 ) than that of patients with T2 stage (7.98 ± 1.10) too.Among 40 patients, all patients showed positive expression of Glut1 and HK-Ⅱ , and the positive expression rate of Glut1 and HK-Ⅱ was ( 45.2 ± 10.9 )% and ( 68.3 ±9.5)%, respectively.The positive expression rate of Glut1 was (38.4 ±8.1)% in T1 stage and (49.7 ±12.6)% in T2 stage, which displayed no difference (x2 =40.58, P＞0.05), but the HK-Ⅱ positive expression rate showed significant difference (x2 =58.71, P＜0.05) between T1 stage (60.1 ±11.1)% and T2 stage (77.9 ± 14.7 )%.The correlation analysis indicated that there was low-degree positive correlation (r =0.369, P=0.019) between the SUVmax and Glut1 expression, and there was medium-degree positive correlation (r = 0.549, P = 0.001 ) between the SUVmax and HK-Ⅱ expression.Conclusion Expression of Glut1 and HK-Ⅱ was positively correlated with FDG uptake in patients with early-stage nasopharyngeal carcinoma.
