肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
7期
473-475
,共3页
食管肿瘤%细胞凋亡%DNA%流式细胞术
食管腫瘤%細胞凋亡%DNA%流式細胞術
식관종류%세포조망%DNA%류식세포술
Esophageal neoplasms%Apoptosis%DNA%Flow cytometry
目的 探讨食管上皮癌变过程中多个相关的细胞凋亡调控因子的表达状况及其意义.方法 应用碘化丙啶染色和间接免疫荧光标记方法,采用流式细胞术对60例食管癌组织及相应的癌旁组织进行定量检测.结果 bcl-2、c-FLIP基因蛋白在食管癌中的表达皆显著高于癌旁组织,两者比较差异有统计学意义(P<0.01);Fadd、Caspase-8和Caspase-3蛋白在食管癌中的表达皆显著低于癌旁组织,两者比较差异有统计学意义(P<0.01).基因相关性比较结果显示,Fadd与Caspase-8基因蛋白表达之间呈正相关关系(P<0.01),与正常黏膜组织和不典型增生组织相比,癌组织中DNA含量明显增高,异倍体细胞显著增加.结论 细胞凋亡的级联调控机制c-FLIP-Fadd-Caspase-8-Caspase-3-bcl-2在食管上皮癌变过程中起着重要作用.食管上皮癌变过程中,DNA含量及异倍体率增加.
目的 探討食管上皮癌變過程中多箇相關的細胞凋亡調控因子的錶達狀況及其意義.方法 應用碘化丙啶染色和間接免疫熒光標記方法,採用流式細胞術對60例食管癌組織及相應的癌徬組織進行定量檢測.結果 bcl-2、c-FLIP基因蛋白在食管癌中的錶達皆顯著高于癌徬組織,兩者比較差異有統計學意義(P<0.01);Fadd、Caspase-8和Caspase-3蛋白在食管癌中的錶達皆顯著低于癌徬組織,兩者比較差異有統計學意義(P<0.01).基因相關性比較結果顯示,Fadd與Caspase-8基因蛋白錶達之間呈正相關關繫(P<0.01),與正常黏膜組織和不典型增生組織相比,癌組織中DNA含量明顯增高,異倍體細胞顯著增加.結論 細胞凋亡的級聯調控機製c-FLIP-Fadd-Caspase-8-Caspase-3-bcl-2在食管上皮癌變過程中起著重要作用.食管上皮癌變過程中,DNA含量及異倍體率增加.
목적 탐토식관상피암변과정중다개상관적세포조망조공인자적표체상황급기의의.방법 응용전화병정염색화간접면역형광표기방법,채용류식세포술대60례식관암조직급상응적암방조직진행정량검측.결과 bcl-2、c-FLIP기인단백재식관암중적표체개현저고우암방조직,량자비교차이유통계학의의(P<0.01);Fadd、Caspase-8화Caspase-3단백재식관암중적표체개현저저우암방조직,량자비교차이유통계학의의(P<0.01).기인상관성비교결과현시,Fadd여Caspase-8기인단백표체지간정정상관관계(P<0.01),여정상점막조직화불전형증생조직상비,암조직중DNA함량명현증고,이배체세포현저증가.결론 세포조망적급련조공궤제c-FLIP-Fadd-Caspase-8-Caspase-3-bcl-2재식관상피암변과정중기착중요작용.식관상피암변과정중,DNA함량급이배체솔증가.
Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.
