CAJ | 학술논문

应用普通电镜和DGD去包埋技术,研究了烟草花粉母细胞中的细胞融合现象及细胞融合过程中细胞骨架的变化.观察发现,处于凝线期的花粉母细胞,其内含物,包括细胞器和染色质,主要通过胞质通道向相邻细胞发生转移.DGD去包埋观察发现,花粉母细胞中核骨架与细胞质内及胞间连丝和胞质通道内胞质骨架连接成一个整体.在整个细胞融合过程中,均有核骨架纤维与染色质相连.本文讨论了细胞骨架在细胞融合过程中的作用.
응용보통전경화DGD거포매기술,연구료연초화분모세포중적세포융합현상급세포융합과정중세포골가적변화.관찰발현,처우응선기적화분모세포,기내함물,포괄세포기화염색질,주요통과포질통도향상린세포발생전이.DGD거포매관찰발현,화분모세포중핵골가여세포질내급포간련사화포질통도내포질골가련접성일개정체.재정개세포융합과정중,균유핵골가섬유여염색질상련.본문토론료세포골가재세포융합과정중적작용.
Conventional transmission electron microscopy and DGD embedment-free technique were used toinvestigate cytomixis and the distribution of cellular skeleton filaments in pollen mother cells (PMC) of to-bacco ( N. tobacum). It was observed that cellular contents, including chromatins and organelles, migrat-ed in PMCs through cytoplasmic channels, mainly at the stage of synizesis in meiotic prophase Ⅰ . FromDGD embedment-free observation, the nuclear matrix, the cytoskeleton in cytoplasm, as well as in plas-modesmata and cytoplasmic channels, connected the PMCs into an entity. The chromatins were in intimateconnection with cellular skeleton filaments, even during the process of migration. The role of cellularskeleton in the process of cytomixis was also discussed.