生物可降解材料聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物与人脐静脉内皮细胞的相容性
생물가강해재료취을이순-취유산-취곡안산삼감단공취물여인제정맥내피세포적상용성
Cytocompatibility of biodegradable poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-glutamic acid) tri-block copolymer with human umbilical vein endothelial cells
  • 万方数据
    中国组织工程研究与临床康复 중국조직공정연구여림상강복
    JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
    2008年 10期 1950-1953 ,共4页

    刘斌%宋显晶%刘婕妤%姜锋%史永峰%双东思%李智博

    류빈%송현정%류첩여%강봉%사영봉%쌍동사%리지박

    细胞相容性%聚乙二醇-聚乳酸-聚谷氨酸%内皮细胞%生物相容性材料 細胞相容性%聚乙二醇-聚乳痠-聚穀氨痠%內皮細胞%生物相容性材料
    세포상용성%취을이순-취유산-취곡안산%내피세포%생물상용성재료
    背景:聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片材料在构建组织工程支架上具有良好的应用前景,内皮细胞能否在该材料上存活和稳定生长将直接影响其作为载内皮细胞载体支架表面可降解材料的应用.目的:观察聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物与人脐静脉内皮细胞的相容性.设计;随机对照观察.单位:吉林大学第二临床医学院.材料:本实验于2006-02/10于吉林大学基础医学院病理生理教研室完成.长约20 cm脐带来自吉林大学第二医院妇产科1名正常足月分娩的新生儿,标本采集经新生儿家属知情同意,本实验经过医院伦理委员会批准.聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片由中国科学院长春应用化学研究所提供.倒置显微镜及相差显微镜为日本Olympus公司产品.方法:将从脐带中分离培养的稳态生长的人脐静脉内皮细胞接种于聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片上培养作为实验组,对照组为未放聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片的培养皿.①以相差显微镜下观察细胞生长情况评价人脐静脉内皮细胞与聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片的细胞相容性.②采用MTT法检测细胞种植后1,3,5及7 d的细胞增殖指数.主要观察指标:①人脐静脉内皮细胞与聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片的细胞相容性.②细胞种植后1,3,5及7 d的细胞增殖指数.结果:①人脐静脉内皮细胞与聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片的细胞相容性:相差显微镜下种植在聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片上的细胞4～6 h后即开始贴壁、伸展,3 d后细胞开始呈集落样生长,生长较快,5 d后细胞集落开始融合,呈现特征性的鹅卵石样形态,经多次传代后,细胞呈长梭形或多角形,与对照组相比无明显差异.扫描电镜下观察在聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物膜片上培养15 d的人脐静脉内皮细胞嵌入膜片间隙生长,但没有连接成片铺在膜片上.②细胞增殖指数:MTT法检测结果显示实验组及对照组实验后1,3,5及7 d的细胞增殖指数差异无显著性意义(P＞0.05).结论:内皮细胞在聚乙二醇-聚乳酸-聚谷氨酸三嵌段共聚物上生长良好,两者具有良好的细胞相容性.
    배경:취을이순-취유산-취곡안산삼감단공취물막편재료재구건조직공정지가상구유량호적응용전경,내피세포능부재해재료상존활화은정생장장직접영향기작위재내피세포재체지가표면가강해재료적응용.목적:관찰취을이순-취유산-취곡안산삼감단공취물여인제정맥내피세포적상용성.설계;수궤대조관찰.단위:길림대학제이림상의학원.재료:본실험우2006-02/10우길림대학기출의학원병리생리교연실완성.장약20 cm제대래자길림대학제이의원부산과1명정상족월분면적신생인,표본채집경신생인가속지정동의,본실험경과의원윤리위원회비준.취을이순-취유산-취곡안산삼감단공취물막편유중국과학원장춘응용화학연구소제공.도치현미경급상차현미경위일본Olympus공사산품.방법:장종제대중분리배양적은태생장적인제정맥내피세포접충우취을이순-취유산-취곡안산삼감단공취물막편상배양작위실험조,대조조위미방취을이순-취유산-취곡안산삼감단공취물막편적배양명.①이상차현미경하관찰세포생장정황평개인제정맥내피세포여취을이순-취유산-취곡안산삼감단공취물막편적세포상용성.②채용MTT법검측세포충식후1,3,5급7 d적세포증식지수.주요관찰지표:①인제정맥내피세포여취을이순-취유산-취곡안산삼감단공취물막편적세포상용성.②세포충식후1,3,5급7 d적세포증식지수.결과:①인제정맥내피세포여취을이순-취유산-취곡안산삼감단공취물막편적세포상용성:상차현미경하충식재취을이순-취유산-취곡안산삼감단공취물막편상적세포4～6 h후즉개시첩벽、신전,3 d후세포개시정집락양생장,생장교쾌,5 d후세포집락개시융합,정현특정성적아란석양형태,경다차전대후,세포정장사형혹다각형,여대조조상비무명현차이.소묘전경하관찰재취을이순-취유산-취곡안산삼감단공취물막편상배양15 d적인제정맥내피세포감입막편간극생장,단몰유련접성편포재막편상.②세포증식지수:MTT법검측결과현시실험조급대조조실험후1,3,5급7 d적세포증식지수차이무현저성의의(P＞0.05).결론:내피세포재취을이순-취유산-취곡안산삼감단공취물상생장량호,량자구유량호적세포상용성.
    BACKGROUND: Poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-glutamic acid) (PEG-PLA-PGL) tri-block copolymers have good applied foreground in constructing tissue engineering scaffold materials. Whether endothelial cells survive and grow on the materials has a direct influence on the application as a biodegradable material for the scaffold of endothelial cell vector.OBJECTIVE: To explore the cytocompatibility of PEG-PLA-PGL tri-block copolymers with human umbilical vein endothelial cells (HUVECs).DESIGN: Randomized control observation.SETTING: the Second Hospital of Jilin University.MATERIALS: The experiment was carried out in the Department of Pathobiology, School of Basic Medical Sciences, Jilin University from February to October in 2006. Human umbilical cord about 20 cm length came from one neonatal infant who was delivered normally after enough months in the Department of Gynecology and Obstetrics, the Second Hospital of Jilin University. Human umbilical cord was sampled in the informed consents of the infant's family member. The experimentation was authorized by the medical ethic committee of the hospital. PEG-PLA-PGL membranes were provided by Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Inverted microscope and phase-contrast microscope were bought from Olympus Company (Japan).METHODS: HUVECs cultivated and grew steadily, were inoculated onto PEG-PLA-PGL membranes, serving as the experiment group. While the culture medium without PEG-PLA-PGL membranes were taken as the control group.①Cytocompatibility of PEG-PLA-PGL membranes with HUVECs was evaluated by observing cellular growth through phase-contrast microscope.②The proliferation index of cells was detected by MTT method in 1, 3, 5 and 7 days after inoculation.MAIN OUTCOME MEASURES: ①Cytocompatibility of PEG-PLA-PGL membranes with HUVECs;②The proliferation index of cells in l, 3, 5 and 7 days after inoculationRESULTS: ①Cytocompatibility of PEG-PLA-PGL membranes with HUVECs: The observation result of phase contrast microscopy showed that, endothelial cells planted on the PEG-PLA-PGL membranes began to attach and stretch after being planted 4-6 hours. Three days later, cells grew in colonies rapidly, after 5 days, colonies began to fuse and seemed like cobble-stone. The cells were shuttle or polygon in shape after passages. There were no significant differences between the experiment and control group. Cells cultured on PEG-PLA-PGL membranes for 15 days grew in inserts with membranes, but they didn't grow into patches through scanning electron microscope.②The proliferation index of cells: No significant differences of the proliferation index of cells were detected by MTT method in 1, 3, 5 and 7 days after inoculation between experiment group and control group (P＞0.05).CONCLUSION: Endothelial cells grow well in PEG-PLA-PGL membranes, and the two have good cytocompatibility.
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