国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
1期
14-15,19
,共3页
肝炎抗体,乙型%酶联免疫吸附测定%标本%定性研究
肝炎抗體,乙型%酶聯免疫吸附測定%標本%定性研究
간염항체,을형%매련면역흡부측정%표본%정성연구
Hepatitis B antibodies%Enzyme-linked immunosorbent assay%Samples%Qualitative research
目的 探讨乙肝病毒表面抗体定性测定S/CO(标本吸光度/临界值)值与定量测定值的相关性.方法 时间分辨荧光免疫分析法(TRF)及酶联免疫吸附试验(ELISA)检测不同浓度的血清,绘制标准曲线,建立方程.结果 S/CO值与定量测定值呈曲线正相关,转换方程式为Y=8.911 8×e0.177 4X.S/CO计算值与实测值组内相关系数为0.934.结论 乙肝病毒表面抗体含量可通过定性S/CO值推算,适用于基层未开展乙肝标志物定量检测的实验室应用.
目的 探討乙肝病毒錶麵抗體定性測定S/CO(標本吸光度/臨界值)值與定量測定值的相關性.方法 時間分辨熒光免疫分析法(TRF)及酶聯免疫吸附試驗(ELISA)檢測不同濃度的血清,繪製標準麯線,建立方程.結果 S/CO值與定量測定值呈麯線正相關,轉換方程式為Y=8.911 8×e0.177 4X.S/CO計算值與實測值組內相關繫數為0.934.結論 乙肝病毒錶麵抗體含量可通過定性S/CO值推算,適用于基層未開展乙肝標誌物定量檢測的實驗室應用.
목적 탐토을간병독표면항체정성측정S/CO(표본흡광도/림계치)치여정량측정치적상관성.방법 시간분변형광면역분석법(TRF)급매련면역흡부시험(ELISA)검측불동농도적혈청,회제표준곡선,건립방정.결과 S/CO치여정량측정치정곡선정상관,전환방정식위Y=8.911 8×e0.177 4X.S/CO계산치여실측치조내상관계수위0.934.결론 을간병독표면항체함량가통과정성S/CO치추산,괄용우기층미개전을간표지물정량검측적실험실응용.
Objective To study the relativity of S/CO qualitative determination of hepatitis B surface antibody and quantitative assay.Methods The different concentrations of sera were determined with time-resolved fluorescence immunoassay and enzyme-linked immunosorbent assay. The standard curve was drawn and the equation was established.Results In the curve, S/CO level was positively correlative to the quantitative detection value, and the conversion equation was Y=8.911 8×e0.177 4X.The intraclass correlation coefficient of S/CO calculating level and quantitative value was 0.934.Conclusion The content of hepatitis B surface antibody can be estimated by S/CO value. It can be used in basic-level laboratories which don′t undertake the quantitative determination of HBV markers.
