CAJ | 학술논문

通过对三个不同来源的纤维素酶进行复配以提高纤维素酶的水解效率,以玉米秸秆酶水解液中还原糖浓度为评价指标,采用混料试验设计优选纤维素酶的复配条件并建立数学拟合方程.结果表明:最佳的酶复配条件为泽生纤维素酶与杰能科纤维素酶体积比1:1时纤维素水解效率最高,比使用单一纤维素酶效率提高了39.5%.利用混料试验设计可以对纤维素酶复配条件进行优化.
통과대삼개불동래원적섬유소매진행복배이제고섬유소매적수해효솔,이옥미갈간매수해액중환원당농도위평개지표,채용혼료시험설계우선섬유소매적복배조건병건립수학의합방정.결과표명:최가적매복배조건위택생섬유소매여걸능과섬유소매체적비1:1시섬유소수해효솔최고,비사용단일섬유소매효솔제고료39.5%.이용혼료시험설계가이대섬유소매복배조건진행우화.
Three different commercial cellulase preparations were used to prepare complex enzyme to increase the hydrolysis efficiency of cellulase. The formulation of cellulose complex was optimized by mixture design to attain the maximum concentra- tion of reduced sugar in hydrolysate solution and a mathematical model describing the relationship of concentration of reduced sugar with volumes of mixed cellulases was developed. A mixture of Zesheng cellulase and Genencor cellulase at a volume ratio of 1:1 resulted in the maximum hydrolysis efficiency higher than that of individual cellulase by 39.5%. Mixture design is available for the optimization of cellulase complex formulation.