广西植物
廣西植物
엄서식물
GUIHAIA
2009年
6期
768-773
,共6页
丁春霞%施国新%徐勤松%杨海燕%许晔%徐小颖
丁春霞%施國新%徐勤鬆%楊海燕%許曄%徐小穎
정춘하%시국신%서근송%양해연%허엽%서소영
水鳖%Pb~(2+)胁迫%抗氧化系统%超微结构
水鱉%Pb~(2+)脅迫%抗氧化繫統%超微結構
수별%Pb~(2+)협박%항양화계통%초미결구
Hydrocharis dubia%Pb~(2+) stress%antioxidant system%ultrastructure
研究了不同浓度Pb~(2+)胁迫(0、10、20、30和50 mg·L~(-1))对水鳖叶绿素含量、可溶性蛋白含量、抗氧化系统、活性氧产生速率和细胞超微结构的影响.结果表明:随着Pb~(2+)浓度的增加,水鳖叶片总叶绿素、叶绿素a/b值、可溶性蛋白含量和CAT活性逐渐下降;SOD、POD、APX、GR活性、抗坏血酸(AsA)和谷胱甘肽(GSH)含量先升后降;O_2~- ·产生速率、H_2O_2、丙二醛(MDA)和可溶性糖含量呈上升趋势;不连续聚丙烯酰胺凝胶电泳(SDS-PAGE)显示,Pb~(2+)处理后,分子量为16.3kDa、55.0kDa的多肽含量减少,高浓度处理下分子量为23.1kDa、75.8kDa、94.0kDa的多肽条带消失;电镜观察发现,Pb~(2+)对水鳖叶片细胞的叶绿体、线粒体和细胞核造成严重损伤.Pb~(2+)破坏了水鳖正常代谢所需的生理和结构基础,打乱了代谢平衡,最终导致植物的衰亡.
研究瞭不同濃度Pb~(2+)脅迫(0、10、20、30和50 mg·L~(-1))對水鱉葉綠素含量、可溶性蛋白含量、抗氧化繫統、活性氧產生速率和細胞超微結構的影響.結果錶明:隨著Pb~(2+)濃度的增加,水鱉葉片總葉綠素、葉綠素a/b值、可溶性蛋白含量和CAT活性逐漸下降;SOD、POD、APX、GR活性、抗壞血痠(AsA)和穀胱甘肽(GSH)含量先升後降;O_2~- ·產生速率、H_2O_2、丙二醛(MDA)和可溶性糖含量呈上升趨勢;不連續聚丙烯酰胺凝膠電泳(SDS-PAGE)顯示,Pb~(2+)處理後,分子量為16.3kDa、55.0kDa的多肽含量減少,高濃度處理下分子量為23.1kDa、75.8kDa、94.0kDa的多肽條帶消失;電鏡觀察髮現,Pb~(2+)對水鱉葉片細胞的葉綠體、線粒體和細胞覈造成嚴重損傷.Pb~(2+)破壞瞭水鱉正常代謝所需的生理和結構基礎,打亂瞭代謝平衡,最終導緻植物的衰亡.
연구료불동농도Pb~(2+)협박(0、10、20、30화50 mg·L~(-1))대수별협록소함량、가용성단백함량、항양화계통、활성양산생속솔화세포초미결구적영향.결과표명:수착Pb~(2+)농도적증가,수별협편총협록소、협록소a/b치、가용성단백함량화CAT활성축점하강;SOD、POD、APX、GR활성、항배혈산(AsA)화곡광감태(GSH)함량선승후강;O_2~- ·산생속솔、H_2O_2、병이철(MDA)화가용성당함량정상승추세;불련속취병희선알응효전영(SDS-PAGE)현시,Pb~(2+)처리후,분자량위16.3kDa、55.0kDa적다태함량감소,고농도처리하분자량위23.1kDa、75.8kDa、94.0kDa적다태조대소실;전경관찰발현,Pb~(2+)대수별협편세포적협록체、선립체화세포핵조성엄중손상.Pb~(2+)파배료수별정상대사소수적생리화결구기출,타란료대사평형,최종도치식물적쇠망.
Hydrocharis dubia individuals were transplanted and cultured under different concentrations of Pb~(2+) stress (0,10,20,30 and 50 mg·L~(-1)) for 7 days. After that,the chlorophyll content,soluble protein content,antioxidant system,active oxygen generation and ultrastructural changes in leaves were investigated. The results showed that with increasing concentration of Pb~(2+),the chlorophyll content,chlorophyll a/b,soluble protein content and CAT activity decreased;SOD,POD,APX,GR activities,AsA and GSH content increased at first and then decreased;O_2~- · generation rate,H_2O_2,MDA and soluble sugar content increased; SDS-PAGE revealed that Pb~(2+) reduced the amounts of polypeptides with apparent molecular weights 16.3kDa and 55.0kDa,while three polypeptides with apparent molecular weights 23.1kDa,75.8kDa and 94.0kDa disappeared with the increasing of Pb~(2+) concentration;transmission electron microscopy observation indicated that the higher Pb~(2+) concentration,the more serious damages the chloroplast,mitochondria and nucleus suffered. It was concluded that physiological and structural foundations in the normal metabolism of Hydrochairs dubia would be destructed by Pb~(2+) stresses,leading to the disorder of metabolic balance and finally causing the death of the plant.
