CAJ | 학술논문

使用自制的包被原和抗萘多克隆兔抗体,建立了测定环境水体中痕量萘的间接竞争酶联免疫吸附法,并优化了实验条件.工作曲线线性方程为I=8.394logρ+50.21(I为抑制率,ρ为萘的质量浓度),线性范围10~(-3)～10 μg/L内的相关系数为0.985 6,方法灵敏度为0.944 μg/L,检出限为0.250 ng/L,回收率为86.00%～106.00%,批间误差为5.00%～15.00%,批内误差为2.00%～8.00%,与四种结构类似物的交叉率均小于14.0%.
사용자제적포피원화항내다극륭토항체,건립료측정배경수체중흔량내적간접경쟁매련면역흡부법,병우화료실험조건.공작곡선선성방정위I=8.394logρ+50.21(I위억제솔,ρ위내적질량농도),선성범위10~(-3)～10 μg/L내적상관계수위0.985 6,방법령민도위0.944 μg/L,검출한위0.250 ng/L,회수솔위86.00%～106.00%,비간오차위5.00%～15.00%,비내오차위2.00%～8.00%,여사충결구유사물적교차솔균소우14.0%.
A new enzyme-linked immunosorbent assay (ELISA) method for determination of trace naphthalene (NA) in environmental water bodies was developed using self-made coating antigen and rabbit polyclonal anti-naphthalene antibodies, and its operation conditions were optimized. The regression equation of the standard ELISA inhibition curve is I=8.394×logρ+50.21 (I for inhibition rate and ρ for NA mass concentration). The linearity range is 10~(-3)-10 μg/L. The correlation coefficient is 0.985 6. The sensitivity is 0.944 μg/L. The detection limit is 0.250 ng/L. The recovery is 86.00%-106.00%. The relative standard deviations of within-run and between-run are 2.00%-8.00%, 5.00%-15.00%, respectively. The cross reaction rates with four structurally analogues are all below 14.0%.