CAJ | 학술논문

在单因素试验基础上,采用正交试验对阳荷蛋白质的提取工艺条件进行筛选,得出最优提取条件:在60℃条件下,用40倍体积于阳荷粉的pH9.0的磷酸缓冲液恒温提取60min.同时对阳荷中锌赋存状态进行研究,结果表明,在供试阳荷中,锌以有机态为主,占总锌含量80%以上,蛋白锌占有机锌65%以上.
재단인소시험기출상,채용정교시험대양하단백질적제취공예조건진행사선,득출최우제취조건:재60℃조건하,용40배체적우양하분적pH9.0적린산완충액항온제취60min.동시대양하중자부존상태진행연구,결과표명,재공시양하중,자이유궤태위주,점총자함량80%이상,단백자점유궤자65%이상.
The optimal extraction process of protein from Zingiber striolatum determined using single factor method coupled with orthogonal array design was that Zingiber striolatum powder was extracted with PBS(pH 9.0)at 60 ℃ for 60 minutes with a solid-to-solvent ratio of 1:40(g/mL).Additionally,spcciafion analysis of zinc in Zingiber striolatum was carried out.Results revealed that organic zinc constituted a major fraction in Zingiber striolatum,which accounted for above 80% of total zinc and comprised above 65% protein-binding zinc.