CAJ | 학술논문

目的:探讨联合应用选择性环氧合酶-2(cyclooxyenase-2,COX-2)抑制剂NS-398与奥曲肽对人结肠癌细胞增殖和凋亡的影响.方法:体外培养人结肠腺癌Lovo细胞,分别用NS-398(100 μmol/L)和奥曲肽(1 μmol/L)单独及联合处理24、48和72 h后,采用MTT法检测细胞的增殖,透射电镜观察细胞凋亡形态学变化,流式细胞仪检测细胞凋亡率和细胞周期改变,RT-PCR检测COX-2基因的表达.结果:NS-398和奥曲肽对Lovo肿瘤细胞的生长具有明显的抑制作用(P<0.05),且存在时间依赖性,联合应用组抑制效应明显高于单一用药组(P<0.05).透射电镜观察可见典型的细胞凋亡形态改变.NS-398联合奥曲肽诱导Lovo细胞的凋亡率明显高于单一用药组和对照组.细胞周期分析表明,与对照组比较,各处理组S期细胞比例降低,G~0/G_1期细胞比例增高(P<0.05).各处理组均使Lovo细胞COX-2基因mRNA表达下调(P<0.05).结论:NS-398联合奥曲肽可协同抑制人结肠腺癌Lovo细胞增殖并诱导其凋亡,其机制可能与细胞周期阻滞和下调COX-2基因表达有关.
목적:탐토연합응용선택성배양합매-2(cyclooxyenase-2,COX-2)억제제NS-398여오곡태대인결장암세포증식화조망적영향.방법:체외배양인결장선암Lovo세포,분별용NS-398(100 μmol/L)화오곡태(1 μmol/L)단독급연합처리24、48화72 h후,채용MTT법검측세포적증식,투사전경관찰세포조망형태학변화,류식세포의검측세포조망솔화세포주기개변,RT-PCR검측COX-2기인적표체.결과:NS-398화오곡태대Lovo종류세포적생장구유명현적억제작용(P<0.05),차존재시간의뢰성,연합응용조억제효응명현고우단일용약조(P<0.05).투사전경관찰가견전형적세포조망형태개변.NS-398연합오곡태유도Lovo세포적조망솔명현고우단일용약조화대조조.세포주기분석표명,여대조조비교,각처리조S기세포비례강저,G~0/G_1기세포비례증고(P<0.05).각처리조균사Lovo세포COX-2기인mRNA표체하조(P<0.05).결론:NS-398연합오곡태가협동억제인결장선암Lovo세포증식병유도기조망,기궤제가능여세포주기조체화하조COX-2기인표체유관.
OBJECTIVE: To study the effects of selective cyclcoxygenase-2 inhibitor NS-398 combined with octreotide on growth and apoptosis of human colon carcinoma cell. METHODS: Lovo cells were treated with NS-398, octreotide or both. The inhibitory effect on the proliferation of Lovo cells was measured by MTT assay. Morphologic changes were examined by electron microscopy, apoptotic percentage and cell cycle of Lovo cells were measured by flow cytometry. The expression of COX-2 mRNA was detected by RT-PCR. RESULTS: NS-398 and octreotide markedly inhibited cells growth in a time-dependent manner, combined treatment could significantly enhance the inhibitory effect than either alone (P < 0.05) . Apoptotic cells were studied with electron microscope. The apeptotic ratio induced by combined treatment was hihger than other groups. Furthermore, cell cycle analysis showed that S phase cells were decreased and quiescent G~0/G_1 phase cells accumulated (P<0.05) . Compared with control group, the levels of COX-2 mRNA was down-regulated in three experimental groups (P<0.05) . CONCLUSION: NS-398 combined with octreotide could synergistically inhibit growth and induce apoptosis of colon carcinoma cell, which may be attributed to ceU cycle block and decrease in COX-2 mRNA expression.