CAJ | 학술논문

研究一种针对禽畜肉中氯霉素残留量的液质联用分析方法.采用乙酸乙酯萃取样品中的氯霉素,氮吹后用水溶解样品残渣,然后用正己烷除去样品溶液中的脂肪,对样品中的目标物进行净化前处理.以乙腈-体积分数0.1%醋酸溶液作流动相,C_(18)色谱柱对食品中的氯霉素残留进行分离,然后用电喷雾质谱负离子选择离子模式IESI(-)-SIM]对氯霉素进行检测.本方法缩短了色谱分离时间,提高了检测灵敏度和分析结果可靠性,具有准确、快速以及灵敏度高的特点,能够适应大规模样品的快速分析要求.
연구일충침대금축육중록매소잔류량적액질련용분석방법.채용을산을지췌취양품중적록매소,담취후용수용해양품잔사,연후용정기완제거양품용액중적지방,대양품중적목표물진행정화전처리.이을정-체적분수0.1%작산용액작류동상,C_(18)색보주대식품중적록매소잔류진행분리,연후용전분무질보부리자선택리자모식IESI(-)-SIM]대록매소진행검측.본방법축단료색보분리시간,제고료검측령민도화분석결과가고성,구유준학、쾌속이급령민도고적특점,능구괄응대규모양품적쾌속분석요구.
A new analytical approach using HPLC-MS was developed for the determination of chioramphenicol residue in meat.Samples were extracted with ethyl acetate,and the resulting extract was blown to dryness under nitrogen.Subsequently,the residue left was dissolved in water,defatted with n-hexan.Finally,the water phase was harvested and filtrated through 0.2 μm membrane for HPLC-MS analysis.The chromatographic separation was achieved on a C_(18) column with a mobile phase consisting of acetronitrile and 0.1% acetate.ESI(-)-SIM mode was adopted for the detection of chloramphenicol.The developed approach is characterized by short time consumption for chromatographic separation,high sensitivity and good reliability so as to meet the requirements for rapid analysis of large-batch samples.