CAJ | 학술논문

通过组织学方法,对产后1 d、10 d、25 d、45 d、60 d及70 d的棕色田鼠Lasiopodomys mandarinus睾丸和附睾发育进行了观察,以探讨其精子发生特点.结果发现,1 d棕色田鼠的生殖细胞主要是生殖母细胞和前精原细胞;10 d出现大量精原细胞,睾丸间质细胞明显;25 d出现精子细胞;45 d有少量精子出现;60 d和70 d具有各级生精细胞,睾丸生精小管和附睾内出现大量成熟精子.睾丸生精小管管径和生精上皮厚度随日龄增加,于60 d达到最大;附睾管腔直径和附睾上皮厚度也于60 d达到最大.这些结果表明,棕色田鼠在生后45 d左右进入青春期,60 d左右达到性成熟,精子的产生及成熟与附睾的发育同步.
통과조직학방법,대산후1 d、10 d、25 d、45 d、60 d급70 d적종색전서Lasiopodomys mandarinus고환화부고발육진행료관찰,이탐토기정자발생특점.결과 발현,1 d종색전서적생식세포주요시생식모세포화전정원세포;10 d출현대량정원세포,고환간질세포명현;25 d출현정자세포;45 d유소량정자출현;60 d화70 d구유각급생정세포,고환생정소관화부고내출현대량성숙정자.고환생정소관관경화생정상피후도수일령증가,우60 d체도최대;부고관강직경화부고상피후도야우60 d체도최대.저사결과표명,종색전서재생후45 d좌우진입청춘기,60 d좌우체도성성숙,정자적산생급성숙여부고적발육동보.
By using histological methods, postnatal changes in the testes and epididymides from birth to adulthood were examined using mandarin voles (Lasiopodomys mandarinus) of 1, 10, 25, 45, 60, and 70 days of age. Results showed that germinal cells were mainly goncytes and prospermatogonia at day 1; abundant spermatogonia, and Leydig cells were found at day 10; Spermatoon were generated at day 25; a few mature sperm were found at day 45; all kinds of spermatogenic cells emerged and lots of mature sperm were produced in seminferous tuble and epididymis at day 60 and day 70. In addition, the diameter of the seminferous tubule and thickness of the seminiferous epithelium significantly increased with pups'age, reaching the highest level at day 60. Furthermore, the diameter of the epididymis duct cavity and the thickness of the epididymal epithelium reached the highest level at day 60. These results suggest that mandarin voles hit puberty at the 45 days and become sexually mature at 60 days. Sperm were produced and became mature synchronically with the development of the epididymides.