CAJ | 학술논문

目的:检测结肠癌细胞株中两种代谢相关基因DPYD和MTHFR的单核苷酸多态性(single nucleotide polymorphisms,SNPs).方法:培养HT-29及Lovo两株结肠癌细胞,分别提取基因组DNA,PCR扩增DPYD和MTHFR基因相应目的片段,采用基于基质辅助激光解吸电离飞行时间质谱技术进行DPYD因rs1801159(A/G)、rs1801160(G/A)、rs17376848(A/G),MTHF基因rs1801131 (A/C)、rs1801133 (C/T)、rs2274976 (G/A)的SNPs位点检测.结果:两种细胞的DPYD基因3个位点rs1801159、rs1801160及rs17376848基因型均为野生纯合型,即A/A、G/G及A/A型.HT-29细胞MTHFR基因rs1801131和rs1801133、rs2274976位点基因型分别为A/C、C/T杂合子和G/G纯合子,而Lovo细胞此3个位点依次为A/A、T/T纯合子和G/A杂合子.结论:本实验中HT-29及Lovo两种细胞株的DPYD基因所测3个SNP位点基因型均相同,而这两种结肠癌细胞株MTHFR基因所测的各SNP位点基因型均不相同.
목적:검측결장암세포주중량충대사상관기인DPYD화MTHFR적단핵감산다태성(single nucleotide polymorphisms,SNPs).방법:배양HT-29급Lovo량주결장암세포,분별제취기인조DNA,PCR확증DPYD화MTHFR기인상응목적편단,채용기우기질보조격광해흡전리비행시간질보기술진행DPYD인rs1801159(A/G)、rs1801160(G/A)、rs17376848(A/G),MTHF기인rs1801131 (A/C)、rs1801133 (C/T)、rs2274976 (G/A)적SNPs위점검측.결과:량충세포적DPYD기인3개위점rs1801159、rs1801160급rs17376848기인형균위야생순합형,즉A/A、G/G급A/A형.HT-29세포MTHFR기인rs1801131화rs1801133、rs2274976위점기인형분별위A/C、C/T잡합자화G/G순합자,이Lovo세포차3개위점의차위A/A、T/T순합자화G/A잡합자.결론:본실험중HT-29급Lovo량충세포주적DPYD기인소측3개SNP위점기인형균상동,이저량충결장암세포주MTHFR기인소측적각SNP위점기인형균불상동.