中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2009年
5期
456-458
,共3页
石秋艳%姜进克%李倩%刘超%孙惠芳%何俊芳%张国志%张瑞彪
石鞦豔%薑進剋%李倩%劉超%孫惠芳%何俊芳%張國誌%張瑞彪
석추염%강진극%리천%류초%손혜방%하준방%장국지%장서표
促红细胞生成素%脑出血%Fas相关死亡域蛋白%Caspase-8
促紅細胞生成素%腦齣血%Fas相關死亡域蛋白%Caspase-8
촉홍세포생성소%뇌출혈%Fas상관사망역단백%Caspase-8
Erythropoietin%Intracerebral hemorrhage%Fas-associated with death domain protein%Caspase-8
目的 研究大鼠脑出血不同时期Fas相关死亡域蛋白(FADD)和Caspase-8表达及促红细胞生成素(EPO)对其的影响,探讨EPO的脑保护机制.方法 SD雄性大鼠126只随机分为假手术组、脑出血组、EPO干预组各42只,各组又按不同时间点分为术后3、6、12、24、48、72 h和7 d共7个亚组(每个亚组6只).采用自体血脑内注射法建立脑出血动物模型,应用免疫组化方法 检测脑出血后不同时间点血肿周边脑组织中FADD和Caspase-8表达情况.结果 脑出血后3 h FADD和Caspase-8升高[分别为(4.66±0.46)和(15.89±1.81)],48 h达到高峰[分别为(35.88±4.24)和(45.04±3.99)],与脑出血组比较,EPO干预组3 h和48 h的FADD和Caspase-8表达显著减少[分别为(3.92±0.64)和(28.24±1.90)、(13.32±2.01)和(35.08±2.85)].结论 脑出血后血肿周围细胞FADD和Caspase-8表达明显增多,EPO可能通过抑制FADD和Caspase-8的表达,对脑出血后脑组织有保护作用.
目的 研究大鼠腦齣血不同時期Fas相關死亡域蛋白(FADD)和Caspase-8錶達及促紅細胞生成素(EPO)對其的影響,探討EPO的腦保護機製.方法 SD雄性大鼠126隻隨機分為假手術組、腦齣血組、EPO榦預組各42隻,各組又按不同時間點分為術後3、6、12、24、48、72 h和7 d共7箇亞組(每箇亞組6隻).採用自體血腦內註射法建立腦齣血動物模型,應用免疫組化方法 檢測腦齣血後不同時間點血腫週邊腦組織中FADD和Caspase-8錶達情況.結果 腦齣血後3 h FADD和Caspase-8升高[分彆為(4.66±0.46)和(15.89±1.81)],48 h達到高峰[分彆為(35.88±4.24)和(45.04±3.99)],與腦齣血組比較,EPO榦預組3 h和48 h的FADD和Caspase-8錶達顯著減少[分彆為(3.92±0.64)和(28.24±1.90)、(13.32±2.01)和(35.08±2.85)].結論 腦齣血後血腫週圍細胞FADD和Caspase-8錶達明顯增多,EPO可能通過抑製FADD和Caspase-8的錶達,對腦齣血後腦組織有保護作用.
목적 연구대서뇌출혈불동시기Fas상관사망역단백(FADD)화Caspase-8표체급촉홍세포생성소(EPO)대기적영향,탐토EPO적뇌보호궤제.방법 SD웅성대서126지수궤분위가수술조、뇌출혈조、EPO간예조각42지,각조우안불동시간점분위술후3、6、12、24、48、72 h화7 d공7개아조(매개아조6지).채용자체혈뇌내주사법건립뇌출혈동물모형,응용면역조화방법 검측뇌출혈후불동시간점혈종주변뇌조직중FADD화Caspase-8표체정황.결과 뇌출혈후3 h FADD화Caspase-8승고[분별위(4.66±0.46)화(15.89±1.81)],48 h체도고봉[분별위(35.88±4.24)화(45.04±3.99)],여뇌출혈조비교,EPO간예조3 h화48 h적FADD화Caspase-8표체현저감소[분별위(3.92±0.64)화(28.24±1.90)、(13.32±2.01)화(35.08±2.85)].결론 뇌출혈후혈종주위세포FADD화Caspase-8표체명현증다,EPO가능통과억제FADD화Caspase-8적표체,대뇌출혈후뇌조직유보호작용.
Objective To study the protein expressions of Fas-associated death domain protein (FADD) and caspase-8 in rats with intracerebral hemorrhage ,and the effects of erythropoietin tp reveal the mechanism of neu-m-protection by EPO. Methods 126 male SD rats were randomly divided into three groups: Sham-operated group, intracerebral hemorrhage group, and EPO group. Each group was divided into seven subgroups according to the differ-ent time points (3,6,12,24,48,72 h and 7 d). The model of intracerebral hemorrage was established in rats by in-tracerebral injection of autogenous blood. The protein expressions of FADD and caspas-8 in rats tissue around the hemorrhagic and the normal brain tissue were detected by immunohistochemistry. Results The protein expressions of FADD and caspase-8 were increased [(4.66±0.46 ) and ( 15.89±1.81)] at 3 h after intracerebral hemorrhage, and peaked at 48 h [ (35.88±4.24 ) and (45.04±3.99)], the expressions of FADD and caspas-8 in the region around hematoma in EPO group significantly decreased compared with model group[ (3.92±0.64) and (28.24±1.90), (13.32±2.01 ) and (35.08±2.82)] at 3 h and 48 h. Conclusion The protein expressions of FADD and easpase-8 are markedly increased after intracerebral hemorrhage. EPO can protect the neurons by signifi-cantly reducing the expressions of FADD and caspase-8.
