中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2007年
4期
251-254
,共4页
高压氧%低氧%脑水肿
高壓氧%低氧%腦水腫
고압양%저양%뇌수종
Hyperbaric oxygenation%Anoxia%Brain edema
目的 通过测定小鼠低氧暴露后脑组织水、钠、钙含量,观察高压氧(HBO)预处理对小鼠低氧后脑水肿预防作用,通过测定小鼠脑组织伊文思蓝(EB)渗透量,观察对小鼠血脑屏障的影响.方法 48只健康雄性昆明小白鼠,重量22～25(22.35±2.31)g,随机均分为高压氧暴露(HBO)组、高压空气(HBA)组、常压空气(NBA)组和空白对照(BC)组.HBO组、HBA组、NBA组分别暴露于各自的气体环境中,每日暴露2次连续3 d,末次暴露结束后24 h,3组动物连续低氧暴露12 h后出舱.测定小鼠脑组织水、钠、钙含量及EB渗透量.组织含水量测定采用分析天平脑组织干、湿重量比较法,钠、钙含量测定采用脑组织湿式灰化和原子吸收分光光谱法,脑组织EB含量测定采用甲酰胺法.结果 HBA脑组织含水量与NBA组差异无统计学意义;HBO组与HBA组相比,HBO组脑组织含水量、钠、钙含量及EB含量均较低,差异有统计学意义(P＜0.01).结论 高压氧预处理可预防小鼠低氧后脑水肿的发生,而预处理时单纯压力增高不能减轻低氧后脑水肿.
目的 通過測定小鼠低氧暴露後腦組織水、鈉、鈣含量,觀察高壓氧(HBO)預處理對小鼠低氧後腦水腫預防作用,通過測定小鼠腦組織伊文思藍(EB)滲透量,觀察對小鼠血腦屏障的影響.方法 48隻健康雄性昆明小白鼠,重量22～25(22.35±2.31)g,隨機均分為高壓氧暴露(HBO)組、高壓空氣(HBA)組、常壓空氣(NBA)組和空白對照(BC)組.HBO組、HBA組、NBA組分彆暴露于各自的氣體環境中,每日暴露2次連續3 d,末次暴露結束後24 h,3組動物連續低氧暴露12 h後齣艙.測定小鼠腦組織水、鈉、鈣含量及EB滲透量.組織含水量測定採用分析天平腦組織榦、濕重量比較法,鈉、鈣含量測定採用腦組織濕式灰化和原子吸收分光光譜法,腦組織EB含量測定採用甲酰胺法.結果 HBA腦組織含水量與NBA組差異無統計學意義;HBO組與HBA組相比,HBO組腦組織含水量、鈉、鈣含量及EB含量均較低,差異有統計學意義(P＜0.01).結論 高壓氧預處理可預防小鼠低氧後腦水腫的髮生,而預處理時單純壓力增高不能減輕低氧後腦水腫.
목적 통과측정소서저양폭로후뇌조직수、납、개함량,관찰고압양(HBO)예처리대소서저양후뇌수종예방작용,통과측정소서뇌조직이문사람(EB)삼투량,관찰대소서혈뇌병장적영향.방법 48지건강웅성곤명소백서,중량22～25(22.35±2.31)g,수궤균분위고압양폭로(HBO)조、고압공기(HBA)조、상압공기(NBA)조화공백대조(BC)조.HBO조、HBA조、NBA조분별폭로우각자적기체배경중,매일폭로2차련속3 d,말차폭로결속후24 h,3조동물련속저양폭로12 h후출창.측정소서뇌조직수、납、개함량급EB삼투량.조직함수량측정채용분석천평뇌조직간、습중량비교법,납、개함량측정채용뇌조직습식회화화원자흡수분광광보법,뇌조직EB함량측정채용갑선알법.결과 HBA뇌조직함수량여NBA조차이무통계학의의;HBO조여HBA조상비,HBO조뇌조직함수량、납、개함량급EB함량균교저,차이유통계학의의(P＜0.01).결론 고압양예처리가예방소서저양후뇌수종적발생,이예처리시단순압력증고불능감경저양후뇌수종.
Objective Water content,total natrium and calcium content of mice brain were detected tO evaluate prevention effect of hyperbaric oxygen(HBO)exposure on the mice brain edema.Evans blue content of mice brain tissue was detected to investigate the effects of HBO on brain edema.Methods Forty-eight male Kunming mice with weight of 22～25(22.5±2.31)g were randomly divided into 4 groups:HBO group,hyperbaric air(HBA)group,normal baric air(NBA)group,and blank control(BC)group.HBO group,HBA group and NBA group were exposed to designed air environment respectively,twice daily for 3 successive days.Twenty-four hours after the last exposure and under same situation.these 3 groups were exposed to hypomia for 12 hours.Water content of mice brain tissue were detected by means of analytic balance,and dry and wet weight were compared.Measurement of total natrium,total calcium adopted wet calcination of brain tissue and atomic absorption spectroscopical spectroscopy.Measurement of Evans blue(EB)content adopted formamide method. Results Compared with NBA group,water content of brain tissue had no significant statistical difference in HBA group.Compared with HBA group,water content,total natrium,total calcium and EB content of brain tissue were significantly reduced(P＜0.01). Conclusions HBO preconditioning can prevent brain edema causing by acute hypoxia, but increasing pressure alone during air preconditioning can not prevent brain edema.
