CAJ | 학술논문

目的探讨6 Gy γ线照射后不同时间小鼠胸腺组织基因表达转录谱的动态变化.方法应用快速高通量的cDNA基因芯片技术进行研究.结果 ①随照射后时间推移,差异表达基因数目和种类逐渐减少,如照射后1、6、14和28 d差异基因数量分别为878、318、162和2n5;②辐射所诱导的胸腺组织差异表达基因广泛涉及细胞周期、免疫和应激、细胞凋亡、细胞信号转导、转录调节、DNA合成修复和重组、细胞骨架、离子通道和运输、代谢、蛋白翻译和合成、发育、细胞分化多个方面;③照射后对某些重要差异表达基因的分析表明,与细胞周期相关的基因有5个(上调3个:CyclinG、Anxal、Fgf1,下调2个:Cdc2a、Cde25b);与免疫应激相关的5个(上调4个:IL-18、Casp1、IL-15、IL-7,下调1个:Cd28);与细胞凋亡相关的7个(上调4个:Casp1、Anxal、Perp、IL-7,下调3个:Pten、Api5、Fas).结论 6 Gy γ线照射后,小鼠胸腺组织差异表达基因随照射后时间延长不仅涉及多个靶点、多个层次,显示出多样性变化的特点,而且显示出时间上的明显差异;从基因水平上揭示了中等剂量照射小鼠胸腺组织损伤修复和重建基因的变化规律,为辐射免疫损伤与修复的分子机制和防治措施的研究提供了新的启示.
목적 탐토6 Gy γ선조사후불동시간소서흉선조직기인표체전록보적동태변화.방법 응용쾌속고통량적cDNA기인심편기술진행연구.결과 ①수조사후시간추이,차이표체기인수목화충류축점감소,여조사후1、6、14화28 d차이기인수량분별위878、318、162화2n5;②복사소유도적흉선조직차이표체기인엄범섭급세포주기、면역화응격、세포조망、세포신호전도、전록조절、DNA합성수복화중조、세포골가、리자통도화운수、대사、단백번역화합성、발육、세포분화다개방면;③조사후대모사중요차이표체기인적분석표명,여세포주기상관적기인유5개(상조3개:CyclinG、Anxal、Fgf1,하조2개:Cdc2a、Cde25b);여면역응격상관적5개(상조4개:IL-18、Casp1、IL-15、IL-7,하조1개:Cd28);여세포조망상관적7개(상조4개:Casp1、Anxal、Perp、IL-7,하조3개:Pten、Api5、Fas).결론 6 Gy γ선조사후,소서흉선조직차이표체기인수조사후시간연장불부섭급다개파점、다개층차,현시출다양성변화적특점,이차현시출시간상적명현차이;종기인수평상게시료중등제량조사소서흉선조직손상수복화중건기인적변화규률,위복사면역손상여수복적분자궤제화방치조시적연구제공료신적계시.
Objective To investigate the dynamic changes of transcription profiles of mouse thymus gene expression in different times after 6 Gy γ-irradiation.Methods High-flux cDNA microarray technique was used irradiation,the numbers and types of differentially expressed genes were gradually decreased,for instance,the induced differential expression genes were involved in cell cycle,immunity and stress,apeptosis,signal transduction,transcription regulation,DNA synthesis and recombination,cystoskeleton,ion channel and transportation,metabolism,protein translation and synthesis,development and cell differentiation,etc.correlated cell cycle(3 up-regulating:Cyclin G,Anxal,Fgf1 and 2 down-regulating:Cdc2a,Cdc25b),5 genes correlated immune stress(4 up-regulating:IL-18,Casp1,IL-15,IL-7 and 1 down-regulating:Cd28),7 genes correlated apoptosis(4 up-regulating:Caspl,Anxal,Perp,IL-7 and 3 down-regulating:Pten,Api5 and Fas).Conclusions After 6 Gy irradiation,differentially expressed genes in mouse thymus is not only involved in many targets,levels and pathways,but also displayed an obvious difference in times.This reveals the regular pattern of differential expression genes in the process of injury and reconstitution in moderate dose irradiated mouse thymus.