CAJ | 학술논문

目的研究大鼠肝脏卵圆细胞增殖模型中卵圆细胞增殖率的变化,进一步了解卵圆细胞生物学特性.方法采用2-乙酰胺基芴/部分肝切除(2-AAF/PH)的方法建立大鼠肝卵圆细胞增殖模型.免疫荧光双标技术检测肝切除术后不同时间点肝脏石蜡切片中卵圆细胞标志物上皮细胞黏附分子和增殖标志物增殖细胞核抗原的共表达情况,对阳性细胞进行定量计数分析;并采用荧光双标和天狼猩红染色观察此过程中肝星状细胞激活和肝脏胶原沉积的情况.结果术后第2天门静脉区开始出现少量卵圆细胞,到第9天数量达到高峰,此后数量逐步下降.此过程中卵圆细胞的增殖率逐步下降,从第2天的(91.3±1.6)%下降到第12天的(53.6±4.4)%,差异有统计学意义(P＜0.01).激活的肝星状细胞一直伴随卵圆细胞增殖并向肝实质延伸,分泌的胶原形成细胞外基质包绕小管样结构的卵圆细胞.结论 2-AAF/PH模型中卵圆细胞数量达到高峰前,其增殖率已经开始逐渐下降.肝星状细胞可能通过分泌细胞外基质和多种因子严格调控卵圆细胞的增殖.
목적 연구대서간장란원세포증식모형중란원세포증식솔적변화,진일보료해란원세포생물학특성.방법 채용2-을선알기물/부분간절제(2-AAF/PH)적방법건립대서간란원세포증식모형.면역형광쌍표기술검측간절제술후불동시간점간장석사절편중란원세포표지물상피세포점부분자화증식표지물증식세포핵항원적공표체정황,대양성세포진행정량계수분석;병채용형광쌍표화천랑성홍염색관찰차과정중간성상세포격활화간장효원침적적정황.결과 술후제2천문정맥구개시출현소량란원세포,도제9천수량체도고봉,차후수량축보하강.차과정중란원세포적증식솔축보하강,종제2천적(91.3±1.6)%하강도제12천적(53.6±4.4)%,차이유통계학의의(P＜0.01).격활적간성상세포일직반수란원세포증식병향간실질연신,분비적효원형성세포외기질포요소관양결구적란원세포.결론 2-AAF/PH모형중란원세포수량체도고봉전,기증식솔이경개시축점하강.간성상세포가능통과분비세포외기질화다충인자엄격조공란원세포적증식.
Objective To investigate the changes of oval cell proliferation rate in the rat 2-acethlaminofluorene/partial hepatectomy(2-AAF/PH)model.Methods Livers were collected from 2-AAF/PH rats at different time points after hepatectomy.Paraffin sections were investigated by double immunofluorescent staining with confocal microscopy for oval cell marker epithelial cell adhesion molecule and proliferative index proliferating cell nuclear antigen,or epithelial cell adhesion molecule and alphasmooth muscle actin Deposition of matrix in liver tissue was detected by sirius red staining.Results Response of ductular oval cells could be observed in portal area at 2 days after PH,and the number of oval cells reached its peak at 9 days and then gradually declined.Oval cell proliferation rate decreased from (91.3±1.6)% at 2 days after PH to(53.6±4.4)% at 12 days(P＜0.01).In addition,oval cells infiltrating into liver parenchyma were closely associated with activated hepatic stellate cells and extracellular matrix.Conclusions Oval cell proliferation rate starts decreasing before its number reaches a peak in 2-AAF/PH model Hepatic stellate cells probably tightly regulate oval cell number through secreting several factors and producing extracellular matrix.