CAJ | 학술논문

目的研究导管相关性MRSE分离株的分子遗传特征及生物学特征,为导管相关性血流感染的诊断与防控提供依据.方法收集2006年1月至2009年12月间,首都医科大学附属北京同仁医院住院患者30例,其中男20例,女10例.采集全血及导管血标本,分离导管相关性MRSE30株.对所有菌株采用MLST方法进行遗传背景分析;采用PCR方法检测菌株icaA基因(编码与致病性相关的胞间多糖黏附素)携带情况;纸片扩散法进行药物敏感试验.结果 30株MRSE包含15种ST型,17株集中在ST259、ST20、ST2和ST235,并发现4种新ST型,将其上传到http://www.mlst.net数据库中,得到序列号为ST259(6株)、ST260(1株)、ST261(1株)和ST262(1株).其中ST259为本院导管相关性MRSE血流分离株的优势克隆株,3株携带icaA基因.结论 4种新ST型中,部分ST259致病性强,可能成为医院内感染的流行株,对医院内感染的预防和控制造成威胁.
목적 연구도관상관성MRSE분리주적분자유전특정급생물학특정,위도관상관성혈류감염적진단여방공제공의거.방법 수집2006년1월지2009년12월간,수도의과대학부속북경동인의원주원환자30례,기중남20례,녀10례.채집전혈급도관혈표본,분리도관상관성MRSE30주.대소유균주채용MLST방법진행유전배경분석;채용PCR방법검측균주icaA기인(편마여치병성상관적포간다당점부소)휴대정황;지편확산법진행약물민감시험.결과 30주MRSE포함15충ST형,17주집중재ST259、ST20、ST2화ST235,병발현4충신ST형,장기상전도http://www.mlst.net수거고중,득도서렬호위ST259(6주)、ST260(1주)、ST261(1주)화ST262(1주).기중ST259위본원도관상관성MRSE혈류분리주적우세극륭주,3주휴대icaA기인.결론 4충신ST형중,부분ST259치병성강,가능성위의원내감염적류행주,대의원내감염적예방화공제조성위협.
Objective To study the genotypic and biological characteristics of catheter-related MRSE isolates and to further provide information for the diagnosis and prevention of catheter-related bloodstream infection. Methods Thirty strains of catheter-related MRSE isolates were collected from venosus blood and whole blood of 30 inpatients including 20 males and 10 females from Beijing Tongren Hospital, Capital Medical University from January 2006 to December 2009. The genetic features of these strains were determined by MLST. PCR was used to detect the icoA gene (encoding the polysaccharide intercellular adhesion associated with pathogenicity), and the antimicrobial susceptibility test was detected by disc diffusion test. Results A total of 15 STs were obtained from 30 strains ST259, ST20, ST2 and ST235 were common clones obtained from 17 strains. Four novel STs were found and uploaded to the MLST database (http://www. mlst. net), including ST259 (6 strains), ST260 (1 strain), ST261 (1 strain) and ST262 (1 strain). The ST259 was the dominant clone of catheter-related MRSE isolates in this hospital, and 3 strains carrying icaA gene were detected in this study. Conclusion Some ST259 isolates express high pathogenesis among the four novel STs, which may make them as the pandemic strains in nosocomial infection, and this would increase the difficulty of the prevention and control of nosocomial infection.