CAJ | 학술논문

目的探讨孕鼠早发子痫前期样改变时,高脂饮食对其血脂水平及妊娠结局的影响.方法 C57BL/6J野生型孕鼠皮下注射左硝基精氨酸甲酯建立早中晚不同时段发病的子痫前期样模型(L-NAME组),同时设立相应对照组皮下注射等量生理盐水(NS组).将以上各组孕鼠再分为高脂饲料亚组(HF,n=6)及标准饲料亚组(SC,n=6)饲喂.同法设立apoE-/-孕鼠作为各相应组的基因型对照.进行动物模型鉴定;于孕中、晚期进行标本采集.比较各组血脂水平和妊娠结局.结果 WT及apoE-/-早中期L-NAME组与相应NS组比较,血浆中甘油三脂(TG)、总胆固醇(TC)游离脂肪酸(FFA)升高明显(P＜0.05),其中以早期L-NAME组升高尤为显著(P＜0.05);apoE-/-孕鼠(L-NAME 组及NS组)血浆中TG、TC含量明显高于WT各相应组(P＜0.05),而血中FFA含量差异不明显(P＞0.05);WT及apoE-/-HF亚组血脂水平比SC亚组有不同程度升高,其中以早期HF+L-NAME尤为明显(P＜0.05).WT及apoE-/-早、中期L-NAME组胎盘重量和胎仔重量明显低于相应NS组(P＜0.05),晚期L-NAME组胎盘重最和胎仔重量与相应NS组相比差异无统计学意义(P＞0.05);各apoE-/-组胎盘重量和胎仔重量比相应WT组低;WT及apoE-/-的HF亚组与相应SC亚组比较,胎盘重量和胎仔重量也有不同程度降低,以早期发病的HF+L-NAME组降低尤为明显(P＜0.05).活胎数及吸收胎数在apoE-/-及WT的L-NAME组与NS组间差异明显(P＜0.05),主要表现为早期L-NAME组活胎数降低,吸收胎数增加.结论妊娠期受到不良因素影响越早,发生早发子痫前期样改变时对血脂及妊娠结局的影响越显著;饮食环境因素町以加重不良因素对血脂及妊娠结局的影响;apoE基因缺陷可引起更为不良的血脂水平变化及妊娠结局.
목적 탐토잉서조발자간전기양개변시,고지음식대기혈지수평급임신결국적영향.방법 C57BL/6J야생형잉서피하주사좌초기정안산갑지건립조중만불동시단발병적자간전기양모형(L-NAME조),동시설립상응대조조피하주사등량생리염수(NS조).장이상각조잉서재분위고지사료아조(HF,n=6)급표준사료아조(SC,n=6)사위.동법설립apoE-/-잉서작위각상응조적기인형대조.진행동물모형감정;우잉중、만기진행표본채집.비교각조혈지수평화임신결국.결과 WT급apoE-/-조중기L-NAME조여상응NS조비교,혈장중감유삼지(TG)、총담고순(TC)유리지방산(FFA)승고명현(P＜0.05),기중이조기L-NAME조승고우위현저(P＜0.05);apoE-/-잉서(L-NAME 조급NS조)혈장중TG、TC함량명현고우WT각상응조(P＜0.05),이혈중FFA함량차이불명현(P＞0.05);WT급apoE-/-HF아조혈지수평비SC아조유불동정도승고,기중이조기HF+L-NAME우위명현(P＜0.05).WT급apoE-/-조、중기L-NAME조태반중량화태자중량명현저우상응NS조(P＜0.05),만기L-NAME조태반중최화태자중량여상응NS조상비차이무통계학의의(P＞0.05);각apoE-/-조태반중량화태자중량비상응WT조저;WT급apoE-/-적HF아조여상응SC아조비교,태반중량화태자중량야유불동정도강저,이조기발병적HF+L-NAME조강저우위명현(P＜0.05).활태수급흡수태수재apoE-/-급WT적L-NAME조여NS조간차이명현(P＜0.05),주요표현위조기L-NAME조활태수강저,흡수태수증가.결론 임신기수도불량인소영향월조,발생조발자간전기양개변시대혈지급임신결국적영향월현저;음식배경인소정이가중불량인소대혈지급임신결국적영향;apoE기인결함가인기경위불량적혈지수평변화급임신결국.
Objective To investigate the effects of dietary factors on the pre-eclampsia-like mouse model development at multiple stages of gestation. Methods Pre-eclampsia-like model was established in C57 wild-type (WT) and apoE-/- pregnant mice at early, middle and late gestational stages by injecting nitric oxide synthase (NOS) inhibitor L-arginine methyl ester (L-NAME) subcutaneously. Control groups received normal saline (NS) simultaneously. Each group was subdivided into standard chow subgroup (SC, n =6)and high-fat diet subgroup (HF, n =6). Blood pressure, urinary protein and plasma lipid were measured and fetal outcomes compared.Data were analyzed statistically. Results In early and middle L-NAME subgroups, the plasma concentrations of cholesterol (TC), triglyceride (TG) and free fatty acid (FFA) all increased. And the early L-NAME subgroup in apoE-/- mice was the most remarkable (P ＜ 0. 05 ). In apoE-/- groups ( both L-NAME and NS groups), the plasma concentrations of total TC and TG were higher than those of WT groups ( P ＜ 0. 05 ). But there was no significant difference in FFA between them. The plasma lipid levels of apoE-/- HF + L-NAME group were the highest among all the groups ( P ＜ 0. 05 ). Fetal and placental weights significantly decreased in early and middle L-NAME groups in both apoE-/- and WT mice (P＜0.05). But no significant difference was found between late L-NAME subgroup and NS groups ( P ＞ 0. 05 ). Compared with WT groups, the weights were lower in apoE-/- mice. The fetal and placental weights in HF groups were lower than SC groups and the changes in early HF + L-NAME subgroup in apoE-/- mice were the most remarkable ( P ＜ 0. 05 ). A lower live fetal rate and a higher absorbed fetal rate were found in early L-NAME groups than those of the NS groups ( P ＜ 0. 05 ). Conclusion Pre-eclampsia occurring at an early stage is more likely to have abnormal plasma lipid levels and adverse feto-placental outcomes. High-fat dietary may aggravate the impact of L-NAME pre-eclampsia on pregnancy outcomes at an early gestational stage especially in ApoE-/- mice.