上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2004年
1期
13-18
,共6页
郑海柔%叶正文%苏明申%金亚放
鄭海柔%葉正文%囌明申%金亞放
정해유%협정문%소명신%금아방
酸枣(Zizyphus jujuba Mill)%60Co-γ射线辐照%组织培养%突变
痠棘(Zizyphus jujuba Mill)%60Co-γ射線輻照%組織培養%突變
산조(Zizyphus jujuba Mill)%60Co-γ사선복조%조직배양%돌변
Jujube (Zizyphus jujuba Mill)%Tissue culture%60Coγ-ray irradiation%Mutation
建立了酸枣离体组织培养结合 60Co-γ射线辐照获得突变株的技术.(1)酸枣叶片外植体在含有ZEA 1mg/L,2,4-D 0.5mg/L和NAA0.5mg/L的MS培养基上诱导愈伤组织:经 60Co-γ射线辐照后在含有ZEA 1mg/L和NAA0.5mg/L的MS培养基上诱导幼苗;(2)试管苗用20～30Gy 60Co-γ射线辐照后,在BAP 2mg/L和IBA 0.4mg/L的MS培养基上进行不定苗繁殖.(3)不定苗在含有IBA 1mg/L和IAA0.4mg/L的MS培养基上生根.此外,用200～450Gy 60Co-γ射线辐照种仁,也能获得突变株.
建立瞭痠棘離體組織培養結閤 60Co-γ射線輻照穫得突變株的技術.(1)痠棘葉片外植體在含有ZEA 1mg/L,2,4-D 0.5mg/L和NAA0.5mg/L的MS培養基上誘導愈傷組織:經 60Co-γ射線輻照後在含有ZEA 1mg/L和NAA0.5mg/L的MS培養基上誘導幼苗;(2)試管苗用20～30Gy 60Co-γ射線輻照後,在BAP 2mg/L和IBA 0.4mg/L的MS培養基上進行不定苗繁殖.(3)不定苗在含有IBA 1mg/L和IAA0.4mg/L的MS培養基上生根.此外,用200～450Gy 60Co-γ射線輻照種仁,也能穫得突變株.
건립료산조리체조직배양결합 60Co-γ사선복조획득돌변주적기술.(1)산조협편외식체재함유ZEA 1mg/L,2,4-D 0.5mg/L화NAA0.5mg/L적MS배양기상유도유상조직:경 60Co-γ사선복조후재함유ZEA 1mg/L화NAA0.5mg/L적MS배양기상유도유묘;(2)시관묘용20～30Gy 60Co-γ사선복조후,재BAP 2mg/L화IBA 0.4mg/L적MS배양기상진행불정묘번식.(3)불정묘재함유IBA 1mg/L화IAA0.4mg/L적MS배양기상생근.차외,용200～450Gy 60Co-γ사선복조충인,야능획득돌변주.
We set up the following technique of in vitro culture combined with 60Coγ-ray irradiation to obtain mutants: (1) inducing calli from jujube leaf explants cultured on the MS medium containing ZEA 1mg/L,2,4-D 0.5mg/L and NAA 0.5mg/L and inducing shoots from leaf calli which were irradiated with 60Coγ-ray and then cultured on the MS medium containing ZEA 1mg/L and NAA 0.5mg/L; (2) test-tube seedlings were irradiated with 60Coγ-ray at 20～30Gy dose and then cultured on the MS medium containing BAP 2mg/L and IBA 0.4mg/L for multiplication of adventitious shoots; (3) culturing young adventitious shoots on MS medium containing IBA 1mg/L and IAA 0.4mg/L for rooting. In addition, irradiating jujube kernels with 60Coγ-ray at 200～450 Gy dose could also obtain mutants.
