CAJ | 학술논문

目的:探讨C-Met抑制剂K252a对人晶状体上皮细胞增殖的抑制作用.方法:取原代培养人晶状体上皮细胞加入HGF、HGF+K252a,以DMEM为对照,应用四唑盐法(MTT法)观察K252a对晶状体上皮细胞生长的抑制作用,蛋白质免疫印迹(Western blot)方法检测K252a对Bcl-2,Caspase-3蛋白表达的影响.结果:HGF(50nmol/L)+K252a(30nmol/L)组与对照组光密度值无显著差异(P＞0.05),Bcl-2和Caspase-3的表达水平变化都不明显.结论:c-Met抑制剂K252a对人晶状体上皮细胞增殖有抑制作用.
목적:탐토C-Met억제제K252a대인정상체상피세포증식적억제작용.방법:취원대배양인정상체상피세포가입HGF、HGF+K252a,이DMEM위대조,응용사서염법(MTT법)관찰K252a대정상체상피세포생장적억제작용,단백질면역인적(Western blot)방법검측K252a대Bcl-2,Caspase-3단백표체적영향.결과:HGF(50nmol/L)+K252a(30nmol/L)조여대조조광밀도치무현저차이(P＞0.05),Bcl-2화Caspase-3적표체수평변화도불명현.결론:c-Met억제제K252a대인정상체상피세포증식유억제작용.
AIM: To observe the inhibition of c-Met inhibitor on proliferation of lens epithelial cells (LECs).METHODS: Human's LECs were cultured and hepatocyte growth factor (HGF) and K252a were added to second passage of cells supplied with Dulbecco's modified eagle's medium (DMEM). MTT assay was used to examine the proliferation of LECs, and Western-blot was used to detect the expression change of Bcl-2 and Caspase-3.RESULTS: The photodensity (A) of HGF (50nmol/L) + K252a (30nmol/L) was not significantly different from that of DMEM control (P＞0.05). The expression of Bcl-2 and Caspase-3 were not significantly different from that in the control group.CONCLUSION: K252a, the inhibitor of c-Met, can effectively inhibit the proliferation of LECs.