CAJ | 학술논문

[目的]获得冬枣花药胚性愈伤组织.[方法]以冬枣花药为试材,研究了碳源种类、基本培养基、植物生长调节剂对冬枣花药愈伤组织诱导及增殖的影响.[结果]麦芽糖为诱导冬枣花药愈伤组织的最适碳源,MS为最适基本培养基,1.0 mg/L TDZ为最适诱导与增殖生长调节剂浓度.[结论] 在MS+麦芽糖20 g/L+TDZ 1.0 mg/L的培养基中,冬枣花药愈伤组织诱导率最高,达86.8%.获得的愈伤组织可进行增殖,愈伤组织呈黄绿色颗粒状,为胚性愈伤.
[목적]획득동조화약배성유상조직.[방법]이동조화약위시재,연구료탄원충류、기본배양기、식물생장조절제대동조화약유상조직유도급증식적영향.[결과]맥아당위유도동조화약유상조직적최괄탄원,MS위최괄기본배양기,1.0 mg/L TDZ위최괄유도여증식생장조절제농도.[결론] 재MS+맥아당20 g/L+TDZ 1.0 mg/L적배양기중,동조화약유상조직유도솔최고,체86.8%.획득적유상조직가진행증식,유상조직정황록색과립상,위배성유상.
[Objective] The study aimed to obtain callus from Dongzao (Ziziphus jujuba Mill.).[Method] With anthers of Z.jujuba as test materials, the effects of carbon source kinds, basic medium, and plant growth regulator on the callus induction and propagation of Z.jujuba anthers were studied.[Result]The results indicated that maltose was the optimum carbon source, MS was the optimum basic medium, and 1.0 mg/L TDZ was the optimum concentration of growth regulator for callus induction and propagation.In the medium of MS+20 g/L maltose + 1.0 mg/L TDZ, the callus induction rate was highest(86.8%).The induced callus was proliferated on MS medium supplemented with maltose 20 g/L+TDZ 1.0 mg/L.And the callus was embryogenic callus with kelly and grain-shape.