西南农业学报
西南農業學報
서남농업학보
SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES
2009年
5期
1326-1328
,共3页
尚慧%杨佩文%李明刚%刘树芳%李家瑞%文孟良
尚慧%楊珮文%李明剛%劉樹芳%李傢瑞%文孟良
상혜%양패문%리명강%류수방%리가서%문맹량
放线菌发酵液提取物%稻瘟病%抗菌活性
放線菌髮酵液提取物%稻瘟病%抗菌活性
방선균발효액제취물%도온병%항균활성
Fermentation extracts of actinomycete%Pyricularia grisea%Inhibitive activity
从云南土壤中筛选分离到一株抗水稻稻瘟病菌的放线菌ECO 00008,对该菌株进行摇瓶发酵后采用乙酸乙酯萃取发酵液,测定其提取物(FR、FE、MR、ME)对水稻稻瘟病的抗菌活性.结果表明,萃取物FE和ME对水稻稻瘟病菌孢子萌发和菌丝生长具有较强的抑制活性,对孢子萌发抑制率分别为99.00 %和95.60 %,盆栽保护效果分别为97.61 %和75.07 %.
從雲南土壤中篩選分離到一株抗水稻稻瘟病菌的放線菌ECO 00008,對該菌株進行搖瓶髮酵後採用乙痠乙酯萃取髮酵液,測定其提取物(FR、FE、MR、ME)對水稻稻瘟病的抗菌活性.結果錶明,萃取物FE和ME對水稻稻瘟病菌孢子萌髮和菌絲生長具有較彊的抑製活性,對孢子萌髮抑製率分彆為99.00 %和95.60 %,盆栽保護效果分彆為97.61 %和75.07 %.
종운남토양중사선분리도일주항수도도온병균적방선균ECO 00008,대해균주진행요병발효후채용을산을지췌취발효액,측정기제취물(FR、FE、MR、ME)대수도도온병적항균활성.결과표명,췌취물FE화ME대수도도온병균포자맹발화균사생장구유교강적억제활성,대포자맹발억제솔분별위99.00 %화95.60 %,분재보호효과분별위97.61 %화75.07 %.
One actinomycetes strain ECO 00008 against Pyricularia grisea was isolated from a soil sample collected from Yunnan. The extracts (FR,FE,MR,ME) of the actinomycete was carried out and the fermentation liquid was extracted with acetic ether. The inhibitive activity of the fermentation extracts against Pyricularia grisea were analyzed. The results showed that the fermentation extracts of FE and ME had significant inhibitive activity to mycelium growth and 99.00 %,95.60 % inhibitive activity rate to spore germination and the control efficiency of potted plant was 97.61 %,75.07 % against Pyricularia grisea.