CAJ | 학술논문

针对羊毛抗菌整理问题,利用新型生物交联剂MTG酶,将具有抗菌作用的乳铁蛋白通过生物催化交联的方式固定到羊毛织物上,对羊毛织物进行抗菌整理研究.采用紫外分光光度法测定乳铁蛋白的固定效率,从而探讨乳铁蛋白固定化的主要工艺参数,得出优化工艺条件,即MTG酶用量为30 U/g织物,温度为40℃,时间为2 h,乳铁蛋白质量浓度为5 mg/mL.研究结果表明,在此工艺条件下羊毛织物上乳铁蛋白的固定效率达到9%左右,整理后的羊毛织物对金黄色葡萄球菌的抑菌率达到57.95%,具有抗菌效果.
침대양모항균정리문제,이용신형생물교련제MTG매,장구유항균작용적유철단백통과생물최화교련적방식고정도양모직물상,대양모직물진행항균정리연구.채용자외분광광도법측정유철단백적고정효솔,종이탐토유철단백고정화적주요공예삼수,득출우화공예조건,즉MTG매용량위30 U/g직물,온도위40℃,시간위2 h,유철단백질량농도위5 mg/mL.연구결과표명,재차공예조건하양모직물상유철단백적고정효솔체도9%좌우,정리후적양모직물대금황색포도구균적억균솔체도57.95%,구유항균효과.
In view of wool fabric anti-bacterial finish, a new biological cross-linker MTG (transglutaminase) was used to catalyze the immobilization of lactoferrin onto the wool fabric and the antibacterial properties of the enzyme treated wool fabric were tested. The lactoferrin immobilization efficiency was determined by UV spectrophotometry. Major parameters in immobilization process were discussed. The optimum conditions were as follows: 30 U/g wool fabric of MTG, 40 ℃ of temperature, 2 h of reactive time, 5 mg/mL of lactoferrin. Under the conditions, the lactoferrin immobilization efficiency on wool fabrics was about 9% , the inhibition rate to staphyllo-COCCUS aureus reached 57. 95% and the treated fabric exhibited good antibacterial property.