CAJ | 학술논문

目的:观察缺血性痴呆(VD)大鼠神经行为学及海马CREB的改变,并探讨两者间的关系.方法:采用双侧颈总动脉永久性结扎(2vessels obstroction,2-VO)法建立血管性痴呆模型,8周后采用Morriss水迷宫实验测试大鼠记忆行为,海马HE染色检测神经元损伤,PCR及免疫组化检测海马CA1区CREB表达的变化.结果:模型组与正常组相比,大鼠认知功和神经元损伤程度明显,海马区CREB表达也明显降低(P<0.01).结论:2-VO法成功制作了缺血性痴呆大鼠模型.模型大鼠脑内海马区CREB水平明显下降,其空间学习记忆障碍可能与海马区CREB表达下降有关.
목적:관찰결혈성치태(VD)대서신경행위학급해마CREB적개변,병탐토량자간적관계.방법:채용쌍측경총동맥영구성결찰(2vessels obstroction,2-VO)법건립혈관성치태모형,8주후채용Morriss수미궁실험측시대서기억행위,해마HE염색검측신경원손상,PCR급면역조화검측해마CA1구CREB표체적변화.결과:모형조여정상조상비,대서인지공화신경원손상정도명현,해마구CREB표체야명현강저(P<0.01).결론:2-VO법성공제작료결혈성치태대서모형.모형대서뇌내해마구CREB수평명현하강,기공간학습기억장애가능여해마구CREB표체하강유관.
Objective: To observe the changes of neuroethology and contents of CREB in vascular dementia rat model, and investigate their relationship. Methods: Model group rats were performed permanent bilateral common carotid artery ligation (2-VO). Morris water maze and HE staining were used to detected the neuronal damage in CA1 zone of hippecampus, the contents of CREB were detected by RT- PCR and immunohistochemistry and analyzed with image analysis system after 8 weeks. Results: The cognitive function in model group was obviously impaired ( P < 0.01 ). The contents of CREB in CA1 zone was also decreased observably in model group (P<0.01 ). Conclusion: With the 2-VO, we performed the VD model suecessfully. The expression of CREB in hippocampus of model group rats are decreased remarkably comparing to the normal group rats, which is contributed to the impairment of learning and memory in the rats of model group.