CAJ | 학술논문

目的研究NaAsO2和Na3AsO4对HL-60细胞生长及基因组DNA甲基化的影响。方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。结果①两种无机砷化物对HL-60细胞生长具有抑制作用，特别是高浓度NaAsO2（2、10 μmol/L）和Na3AsO4（100、300 μmol/L）可明显地抑制HL-60细胞的生长（P<0.01)。②随着砷化物浓度的增加，MspⅠ酶解基因组DNA片段的分子量变化不大，但HpaⅡ酶解片段的分子量逐渐变大。结论提示基因组DNA中CCGG核苷酸内侧C的甲基化总的水平有上升趋势。
목적연구NaAsO2화Na3AsO4대HL-60세포생장급기인조DNA갑기화적영향。방법채용세포배양화한제성내절매해분석기술관찰분석세포생장급세포기인조DNA갑기화수평。결과①량충무궤신화물대HL-60세포생장구유억제작용，특별시고농도NaAsO2（2、10 μmol/L）화Na3AsO4（100、300 μmol/L）가명현지억제HL-60세포적생장（P<0.01)。②수착신화물농도적증가，MspⅠ매해기인조DNA편단적분자량변화불대，단HpaⅡ매해편단적분자량축점변대。결론제시기인조DNA중CCGG핵감산내측C적갑기화총적수평유상승추세。
Objective　To study the effects of arsenicals on growth and genomic DNA methylation of HL-60 cells.Methods　Cell culture and methylation-sensitive restriction endonuclease digestion were used.Results　①Arsenicals caused inhibition obviously on HL-60 cells growth at the test concentrations,particularly in　the groups of 2μmol/L,10μmol/L sodium arsenite,and the groups of 100 μmol/L,300 μmol/L sodium arsenate.②The length of HpaⅡrestriction fragments were substantially increased concurrently with the increasing of arsenic doses for treating the HL-60 cells,and no change was observed for MspI cutting bands.Conclusions　Arsenicals can inhibit the growth of HL-60 cells,and cause hypermethylation in internal cytosine-methylation in the CmCGG sequence of the total genomic DNA.