CAJ | 학술논문

目的探索局部应用免疫抑制剂对提高移植胰岛细胞的存活率及改善全身不良反应的影响.方法造模:将链脲霉素按200 mg/kg注射到昆明小鼠腹腔内,48、72 h后测血糖,连续测两次≥20 mol/L作为实验动物糖尿病型.腹腔内移植入胰岛细胞的量为8 000 IE/kg,环孢素用量为1.5 mg/(100 g·d).实验分3组:(1)经胃灌注免疫抑制剂与腹腔内注射微囊化胰岛细胞;(2)单纯腹腔内注射微囊化胰岛细胞;(3)腹腔内注射载有免疫抑制剂的微囊化的活性炭颗粒与微囊化的胰岛细胞.检测移植后大鼠的血糖及病理的变化.结果 1组与3组血糖变化差异无统计学意义(P＞0.05),与2组相比(P＞0.05)差异无统计学意义.局部应用免疫制剂后,胰岛细胞作用时间较对照组长,胰岛周嗣纤维化程度较轻,C肽水平明显高于单纯移植组.结论与经胃灌注应用免疫抑制相比,局部应用缓慢释放免疫抑制剂的活性碳颗粒效果与之相当,但后者可延长移植胰岛细胞的作用时间并可减轻局部反应.
목적 탐색국부응용면역억제제대제고이식이도세포적존활솔급개선전신불량반응적영향.방법 조모:장련뇨매소안200 mg/kg주사도곤명소서복강내,48、72 h후측혈당,련속측량차≥20 mol/L작위실험동물당뇨병형.복강내이식입이도세포적량위8 000 IE/kg,배포소용량위1.5 mg/(100 g·d).실험분3조:(1)경위관주면역억제제여복강내주사미낭화이도세포;(2)단순복강내주사미낭화이도세포;(3)복강내주사재유면역억제제적미낭화적활성탄과립여미낭화적이도세포.검측이식후대서적혈당급병리적변화.결과 1조여3조혈당변화차이무통계학의의(P＞0.05),여2조상비(P＞0.05)차이무통계학의의.국부응용면역제제후,이도세포작용시간교대조조장,이도주사섬유화정도교경,C태수평명현고우단순이식조.결론 여경위관주응용면역억제상비,국부응용완만석방면역억제제적활성탄과립효과여지상당,단후자가연장이식이도세포적작용시간병가감경국부반응.
Objective To explore the local application of immunosuppressant in improving the survival rate of the transplanted islet cells and systemic side effects.Methods The streptozocin of 200 ms/kg was injected into the abdominal cavity of the Wistar rats，the blood sugar was tested after 48，and 72 hours，and the rats with two consecutive measurements ≥20 mol/L were taken as the experimental animal model.The dose of pancreatic islet cells transplanted into the abdominal cavity was 8 000 IE,/kg，and that of cyclosporine dosage was 1.5 mg/(100 g·d).The pancreatic islet cells were divided into three groups：(1)systemic immunosuppressive agents through stomach lavage with the intraperitoneal injection of microencapsulated islet cells；(2)pure intraperitoneal injection of microencapsulated islet cells；(3)intraperitoneal injection microencapsulated activated carbon particles loaded with immunosuppressants，and mieroencapsulated islet cells.Changes of blood glucose and pathological in rats after transplantation were detected.Results The blood glucose of group 3 and group 1 showed no significant difference(P＞0.05)，as well as compared with group 2(P＞0.05).But the local application of immune agents could prolong the effective time of the islet cells and attenuate the fibrotic extent of the surrounding islets when compared with the control group,the C peptide level in applicating immunosuppressive agents group was significantly hisher in the immunosuppressive group than the pure transplantation group.Conclusion Compared with the systemic immune suppression via stomach lavage，local application of slow-release immunosuppressive agents showed the same effects of activated carbon particles，with a prolonged the effective time of islet cell and reduced topical side effects in the latter.