CAJ | 학술논문

目的建立肺动脉瘘伴t(1;3)易位家系永生细胞系,为肺动脉瘘发病及遗传机制的研究提供永久的研究材料.方法用EB病毒(Epstein-Barr virus)转染肺动脉瘘患者外周血B淋巴细胞,在37℃ 5%CO_2培养箱内培养(培养液成分:20%FBS、RPM11640、环胞霉素A和PHA),当细胞增长到汇合状态时传代及冻存.结果所有建立的细胞系冻存后复苏的成功率为100%,经染色体G显带分析未见异常,表明建立细胞系的方法稳定.结论成功地对肺动脉瘘伴t(1;3)易位家系中6个个体建立了永生细胞系.其中2个为肺动脉瘘患者,4个为患者家属正常对照.
목적 건립폐동맥루반t(1;3)역위가계영생세포계,위폐동맥루발병급유전궤제적연구제공영구적연구재료.방법 용EB병독(Epstein-Barr virus)전염폐동맥루환자외주혈B림파세포,재37℃ 5%CO_2배양상내배양(배양액성분:20%FBS、RPM11640、배포매소A화PHA),당세포증장도회합상태시전대급동존.결과 소유건립적세포계동존후복소적성공솔위100%,경염색체G현대분석미견이상,표명건립세포계적방법은정.결론 성공지대폐동맥루반t(1;3)역위가계중6개개체건립료영생세포계.기중2개위폐동맥루환자,4개위환자가속정상대조.
Objective To establish permanent cell lines of fistula of artery family with t(1;3) and offer a method for research on pathogenesis and genetic mechanism of fistula of artery.Methods Peripheral blood lymphocytes of patients was transtected with fistula of artery by Epstein-Barr virus,cultured in 37℃,5% CO_2 incubator (culture medium:20%FBS,RPMI1640,cyclosporin A and PHA).The transfected cells are passaged and frozen in confluence.Results The survival of frozen cells is 100%.There are no abnormality in chromosomes G-banding,which indicates the method is stable and available.Conclusion Successfully establish six individual permanent cell lines,including two patients of fistula of artery with t(1;3) and four control individuals from the family.