CAJ | 학술논문

目的探讨骨形态发生蛋白(rhBMP-2)的聚乳酸聚乙醇酸共聚物(PLGA)体外缓释生物支架对人骨髓间充质干细胞(MSCs)细胞的影响.方法采用粒子沥滤-冷冻干燥复合工艺制备了附载rhBMP-2的PLGA生物支架,并检测了在PLGA的降解过程中rhBMP-2的释药规律;同时分离培养人骨髓间充质干细胞,体外培养后分别接种于附载和未附载rhBMP-2的PLGA支架上.扫描电镜观察不同时间段MSC在支架上的生长情况;MTT法测定细胞增殖情况.结果 rhBMP-2能被包裹进PLGA支架中,而且可以在PLGA支架降解过程中持续释放出来并诱导骨发生.结论骨形态发生蛋白的PLGA复合载体是一种较为理想的新型生物支架.
목적 탐토골형태발생단백(rhBMP-2)적취유산취을순산공취물(PLGA)체외완석생물지가대인골수간충질간세포(MSCs)세포적영향.방법 채용입자력려-냉동간조복합공예제비료부재rhBMP-2적PLGA생물지가,병검측료재PLGA적강해과정중rhBMP-2적석약규률;동시분리배양인골수간충질간세포,체외배양후분별접충우부재화미부재rhBMP-2적PLGA지가상.소묘전경관찰불동시간단MSC재지가상적생장정황;MTT법측정세포증식정황.결과 rhBMP-2능피포과진PLGA지가중,이차가이재PLGA지가강해과정중지속석방출래병유도골발생.결론 골형태발생단백적PLGA복합재체시일충교위이상적신형생물지가.
Objective To assess the feasibility of using biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffold with controlled release of rhBMP-2 and its effect on the osteogenic activity of MSCs in vitro. Methods The PLGA scaffolds with rhBMP-2 sustained release system were made by combination of porogen-leaching and freeze-drying. The drug release kinetics was measured by enzyme-linked immunosorbent assay (ELISA) in vitro. MSCs were isolated from the bone marrow of human and cultured for 3 passages. Then, MSCs were seeded onto PLGA scaffolds. PLGA scaffolds without rhBMP-2 were used as experimental controls . Scanning electron microscopy(SEM) was used to observe the morphology of MSCs. The cell proliferation was determined by MTT assay. Results The rhBMP-2 was encapsulated into PLGA scaffolds and it was found to be continuously released from the scaffolds. The scaffolds have evident enhancing effect on ectopia osteogenesis. Conclusion PLGA scaffold containing rhBMP-2 is a new promising tissue engineering scaffold.