CAJ | 학술논문

目的定量检测慢性乙型肝炎(CHB)、乙型肝炎肝硬化(LC)、肝细胞癌(HCC)患者HBV总DNA(tDNA)、HBV共价闭合环状DNA (cccDNA)和HBsAg,并探讨其特点.方法荧光定量PCR检测21例CHB、23例LC和25例HCC患者外周血和肝组织标本HBV tDNA、HBVcccDNA,化学发光法定量检测外周血HBsAg.正态数据采用ANOVA分析和t检验,相关性分析采用Pearson检验,非正态数据采用秩和检验.结果在CHB、LC和HCC患者中,外周血HBVtDNA分别为(5.38±2.08)、(4.96±1.65)和(4.18±0.91)lg拷贝/mL,肝组织HBV tDNA分别为(7.18±1.91)、(6.51±1.87)和(5.87±1.47)lg拷贝/μg,肝组织HBV cccDNA分别为(3.53±2.03)、(2.63±2.13)和(0.58±1.40)lg拷贝/μg,外周血H BsAg分别为(3.30±0.65)、(3.12±0.52)和(2.60±1.03)lg IU/mL,CHB与HCC患者比较,差异均有统计学意义(t=2.446,P=0.013；t=2.562,P=0.014;t=5.799,P＜0.01；t=2.709,P=0.003),LC与HCC患者肝组织HBVcccDNA及HBsAg定量比较,差异有统计学意义(t=-3.894,P＜0.01；t=-2.237,P=0.023).外周血均未检出HBV cccDNA.HBsAg定量与外周血HBV tDNA(r=-0.290,P=0.016)、肝组织HBV tDNA(r=0.372,P=0.002)及肝组织HBV cccDNA(r=0.378,P=0.001)均有关.结论 HBV tDNA、HBV cccDNA、HBsAg在CHB、LC、HCC患者呈逐渐降低趋势,HBsAg定量与外周血HBV tDNA、肝组织HBV tDNA及肝组织HBV cccDNA有关.
목적 정량검측만성을형간염(CHB)、을형간염간경화(LC)、간세포암(HCC)환자HBV총DNA(tDNA)、HBV공개폐합배상DNA (cccDNA)화HBsAg,병탐토기특점.방법 형광정량PCR검측21례CHB、23례LC화25례HCC환자외주혈화간조직표본HBV tDNA、HBVcccDNA,화학발광법정량검측외주혈HBsAg.정태수거채용ANOVA분석화t검험,상관성분석채용Pearson검험,비정태수거채용질화검험.결과 재CHB、LC화HCC환자중,외주혈HBVtDNA분별위(5.38±2.08)、(4.96±1.65)화(4.18±0.91)lg고패/mL,간조직HBV tDNA분별위(7.18±1.91)、(6.51±1.87)화(5.87±1.47)lg고패/μg,간조직HBV cccDNA분별위(3.53±2.03)、(2.63±2.13)화(0.58±1.40)lg고패/μg,외주혈H BsAg분별위(3.30±0.65)、(3.12±0.52)화(2.60±1.03)lg IU/mL,CHB여HCC환자비교,차이균유통계학의의(t=2.446,P=0.013；t=2.562,P=0.014;t=5.799,P＜0.01；t=2.709,P=0.003),LC여HCC환자간조직HBVcccDNA급HBsAg정량비교,차이유통계학의의(t=-3.894,P＜0.01；t=-2.237,P=0.023).외주혈균미검출HBV cccDNA.HBsAg정량여외주혈HBV tDNA(r=-0.290,P=0.016)、간조직HBV tDNA(r=0.372,P=0.002)급간조직HBV cccDNA(r=0.378,P=0.001)균유관.결론 HBV tDNA、HBV cccDNA、HBsAg재CHB、LC、HCC환자정축점강저추세,HBsAg정량여외주혈HBV tDNA、간조직HBV tDNA급간조직HBV cccDNA유관.
Objective To quantitatively analyze total hepatitis B virus (HBV) DNA (HBV tDNA),covalently closed circular DNA (cccDNA) and HBsAg in patients with chronic hepatitis B (CHB),HBV-related liver cirrhosis (LC) and hepatocellular carcinoma (HCC),and to analyze the characteristics.Methods HBV tDNA and HBV cccDNA in the serum and liver biopsy samples were measured in 21 CHB,23 LC and 25 HCC patients by real-time polymerase chain reaction (PCR) assay. HBsAg titer was measured by chemiluminescence. Normally distributed variables among multiple groups were analyzed by ANOVA and t-test.Correlation between two variables was tested using Pearson correlation analysis.Skewed distribution was tested using Rank sum test.Results In CHB,LC and HCC patients,the serum HBV tDNA levels were (5.38±2.08),(4.96± 1.65) and (4.18 ± 0.91) lg copy/mL,respectively; the intrahepatic HBV tDNA levels in three groups were (7.18±1.91),(6.51±1.87) and (5.87± 1.47) lg copy/ug,respectively; the intrahepatic HBV cccDNA levels were (3.53±2.03),(2.63±2.13) and (0.58± 1.40) lg copy/μg,respectively; the serum HBsAg levels were (3.30±0.65),(3.12±0.52) and (2.60± 1.03) lg IU/mL,respectively.In CHB patients,the serum HBV tDNA,intrahepatic HBV tDNA,HBV cccDNA and HBsAg levels were all significantly higher than those of HCC patients (t=2.446,P=0.013; t=2.562,P=0.014;t=5.799,P＜0.01 ; t=2.709,P=0.003,respectively).However,only intrahepatic HBV cccDNA and HBsAg levels were statistically different between LC and HCC patients (t=-3.894,P＜0.01;t=-2.237,P=0.023,respectively).HBV cccDNA was all negative in the serum of 69 patients.The serum HBsAg level was positively correlated with serum HBV tDNA (r=0.290,P=0.016),intrahepatic HBV tDNA (r=0.372,P =0.002) and intrahepatic HBV cccDNA (r=0.378,P=0.001).Conclusions The levels of HBV tDNA,HBV cccDNA and HBsAg decrease gradually with the disease progression.The serum HBsAg level is positively correlated with serum HBV tDNA,intrahepatic HBV tDNA and intrahepatic HBV cccDNA.