中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
3期
292-295
,共4页
曾彦茹%佘守章%许立新%许学兵
曾彥茹%佘守章%許立新%許學兵
증언여%사수장%허립신%허학병
右美托咪啶%疼痛%中脑%脑导水管%去甲肾上腺素
右美託咪啶%疼痛%中腦%腦導水管%去甲腎上腺素
우미탁미정%동통%중뇌%뇌도수관%거갑신상선소
Dexmedetomidine%Pain%Mesencephalon%Cerebral aqueduct%Norepinephrine
目的 探讨右美托咪啶对切口痛大鼠中脑导水管去甲肾上腺素(NE)释放的影响.方法 成功植入微透析系统的雄性Wistar大鼠24只,采用随机数字表法,将大鼠随机分为4组(n=6),对照组(C组):腹腔注射0.9%生理盐水2 ml,15 min后吸入2%异氟醚;切口痛组(IP组):腹腔注射0.9%生理盐水2 ml,15 min后制备切口痛模型;D组:腹腔注射右美托咪啶30 μg/kg,15 min后制备切口痛模型;拮抗组(DY组):腹腔注射右美托咪啶30 μg/kg和育亨宾0.5 mg/kg,15 min后制备切口痛模型.除DY组外,其余各组于术前30 min(基础状态)、术后4 h内每30 min收集微透析液10μl,DY组于术前30 min、术后30、60 min时收集微透析液,采用高效液相色谱仪和电化学检测器测定微透析液NE浓度;DY组于术前30 min(基础状态)、术后1 h,其余各组于术前30 min(基础状态)、术后1、2、3、4h时测定机械缩足反应阈值(MWT).结果 与C组比较,IP组和DY组术后MWT降低,微透析液NE浓度升高,D组术后微透析液NE浓度升高(P＜0.05),MWT差异无统计学意义(P＞0.05);与IP组比较,D组和DY组术后MWT升高,微透析液NE浓度降低(P＜0.05);与D组比较,DY组术后MWT降低,微透析液NE浓度升高(P＜0.05).结论 右美托咪啶可抑制切口痛大鼠中脑导水管NE的释放,从而产生中枢镇痛作用.
目的 探討右美託咪啶對切口痛大鼠中腦導水管去甲腎上腺素(NE)釋放的影響.方法 成功植入微透析繫統的雄性Wistar大鼠24隻,採用隨機數字錶法,將大鼠隨機分為4組(n=6),對照組(C組):腹腔註射0.9%生理鹽水2 ml,15 min後吸入2%異氟醚;切口痛組(IP組):腹腔註射0.9%生理鹽水2 ml,15 min後製備切口痛模型;D組:腹腔註射右美託咪啶30 μg/kg,15 min後製備切口痛模型;拮抗組(DY組):腹腔註射右美託咪啶30 μg/kg和育亨賓0.5 mg/kg,15 min後製備切口痛模型.除DY組外,其餘各組于術前30 min(基礎狀態)、術後4 h內每30 min收集微透析液10μl,DY組于術前30 min、術後30、60 min時收集微透析液,採用高效液相色譜儀和電化學檢測器測定微透析液NE濃度;DY組于術前30 min(基礎狀態)、術後1 h,其餘各組于術前30 min(基礎狀態)、術後1、2、3、4h時測定機械縮足反應閾值(MWT).結果 與C組比較,IP組和DY組術後MWT降低,微透析液NE濃度升高,D組術後微透析液NE濃度升高(P＜0.05),MWT差異無統計學意義(P＞0.05);與IP組比較,D組和DY組術後MWT升高,微透析液NE濃度降低(P＜0.05);與D組比較,DY組術後MWT降低,微透析液NE濃度升高(P＜0.05).結論 右美託咪啶可抑製切口痛大鼠中腦導水管NE的釋放,從而產生中樞鎮痛作用.
목적 탐토우미탁미정대절구통대서중뇌도수관거갑신상선소(NE)석방적영향.방법 성공식입미투석계통적웅성Wistar대서24지,채용수궤수자표법,장대서수궤분위4조(n=6),대조조(C조):복강주사0.9%생리염수2 ml,15 min후흡입2%이불미;절구통조(IP조):복강주사0.9%생리염수2 ml,15 min후제비절구통모형;D조:복강주사우미탁미정30 μg/kg,15 min후제비절구통모형;길항조(DY조):복강주사우미탁미정30 μg/kg화육형빈0.5 mg/kg,15 min후제비절구통모형.제DY조외,기여각조우술전30 min(기출상태)、술후4 h내매30 min수집미투석액10μl,DY조우술전30 min、술후30、60 min시수집미투석액,채용고효액상색보의화전화학검측기측정미투석액NE농도;DY조우술전30 min(기출상태)、술후1 h,기여각조우술전30 min(기출상태)、술후1、2、3、4h시측정궤계축족반응역치(MWT).결과 여C조비교,IP조화DY조술후MWT강저,미투석액NE농도승고,D조술후미투석액NE농도승고(P＜0.05),MWT차이무통계학의의(P＞0.05);여IP조비교,D조화DY조술후MWT승고,미투석액NE농도강저(P＜0.05);여D조비교,DY조술후MWT강저,미투석액NE농도승고(P＜0.05).결론 우미탁미정가억제절구통대서중뇌도수관NE적석방,종이산생중추진통작용.
Objective To investigate the effect of dexmedetomidine on norepinephrine(NE)release in midbrain periaqueductal gray(PAG)in a rat model of incisional pain.Methods Twenty-four male Wistar rats in which microdialvsis catheter was successfully placed in the ventrolateral region of PAG without complications were randomly divided into 4 groups(n=6 each):group control(group C);group incisional pain(group IP);group dexmetomidine(group D)and group dexmedetomidine+yohimbine(group DY).Incisional pain was induced by an incision made into the plantar surface of left hindpaw in IP,D,DY groups.Dexmedetomidine 30 μg/kg and dexmedetomidine 30 μg/kg+yohimbine 0.5 mg/kg were given intraperitoneally at 15 min before plantar incision in group D and group DY respectively.Mechanical paw withdrawal threshold(MWT)to von Frey filament stimulation was measured at 30 min before(baseline)and 1,2,3,4 h after operation in C,IP,D groups,and at 30 min before(baseline),and 1 h after operation in group DY.Dialysate samples were collected at 30 min before(baseline)and at evcry 30 min after operation for 4 h via cerebral microdialysis catheter for determination of the NE concentration in C,IP,D groups,and at 30 min before(baseline),30,60 min after operation in group DY.Results Incisional pain significantly decreased MWT and increased the NE concentration in dialysate in group IP.Dexmedetomidine premedication significantly inhibited mechanical hyperalgesia and attenuated incisional pain-induced increase in the NE concentration in dialysate in group D.Yohimbine counteracted effects of dexmedetomidine.Conclusion Dexmedetomidine has analgesic effect though inhibition of NE release from PAG.
