中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
6期
371-373
,共3页
张彩萍%季江%林麟%杨毅琴%崔盘根%冯雨苗%岳晓玉%赵亮%曾学思%吴勤学%王洪生
張綵萍%季江%林麟%楊毅琴%崔盤根%馮雨苗%嶽曉玉%趙亮%曾學思%吳勤學%王洪生
장채평%계강%림린%양의금%최반근%풍우묘%악효옥%조량%증학사%오근학%왕홍생
分枝杆菌,结核%抗药性%链霉素抗药性%基因,rpsL
分枝桿菌,結覈%抗藥性%鏈黴素抗藥性%基因,rpsL
분지간균,결핵%항약성%련매소항약성%기인,rpsL
Mycobacterinm tuberculosis%Drug resistance%Streptomycin resistance%Genes,rpsL
目的 探讨结核分枝杆菌皮肤感染的耐药情况,为该病的临床治疗提供参考依据.方法 分离培养的16株分枝杆菌经生化和分子生物学方法鉴定为结核分枝杆菌;采用国内公认的绝对浓度间接法分别检测16株结核分枝杆菌对异烟肼、链霉索、利福平和乙胺丁醇四种药物的耐药情况;对其中2株链霉素耐药临床株进行rpsL基因扩增和DNA测序.结果 16株结核分枝杆菌中,2株对链霉素耐药,对异烟肼、利福平和乙胺丁醇敏感;其余14株对利福平、异烟肼、乙胺丁醇和链霉素均敏感.2株链霉素耐药临床株中,1株rpsL基因43位密码子突变为AAG→AGG;另一株rpsL基凶54位密码子突变为CGC→AC.结论 近5年收治的结核分枝杆菌皮肤感染患者耐药率不高,以链霉素耐药为主.
目的 探討結覈分枝桿菌皮膚感染的耐藥情況,為該病的臨床治療提供參攷依據.方法 分離培養的16株分枝桿菌經生化和分子生物學方法鑒定為結覈分枝桿菌;採用國內公認的絕對濃度間接法分彆檢測16株結覈分枝桿菌對異煙肼、鏈黴索、利福平和乙胺丁醇四種藥物的耐藥情況;對其中2株鏈黴素耐藥臨床株進行rpsL基因擴增和DNA測序.結果 16株結覈分枝桿菌中,2株對鏈黴素耐藥,對異煙肼、利福平和乙胺丁醇敏感;其餘14株對利福平、異煙肼、乙胺丁醇和鏈黴素均敏感.2株鏈黴素耐藥臨床株中,1株rpsL基因43位密碼子突變為AAG→AGG;另一株rpsL基兇54位密碼子突變為CGC→AC.結論 近5年收治的結覈分枝桿菌皮膚感染患者耐藥率不高,以鏈黴素耐藥為主.
목적 탐토결핵분지간균피부감염적내약정황,위해병적림상치료제공삼고의거.방법 분리배양적16주분지간균경생화화분자생물학방법감정위결핵분지간균;채용국내공인적절대농도간접법분별검측16주결핵분지간균대이연정、련매색、리복평화을알정순사충약물적내약정황;대기중2주련매소내약림상주진행rpsL기인확증화DNA측서.결과 16주결핵분지간균중,2주대련매소내약,대이연정、리복평화을알정순민감;기여14주대리복평、이연정、을알정순화련매소균민감.2주련매소내약림상주중,1주rpsL기인43위밀마자돌변위AAG→AGG;령일주rpsL기흉54위밀마자돌변위CGC→AC.결론 근5년수치적결핵분지간균피부감염환자내약솔불고,이련매소내약위주.
Objective To test the drug susceptibility of 16 Mycobactena tuberculosis isolates from patients with cutaneous tuberculosis,and to provide a basis for the treatment of this entity.Methods Sixteen strains of mycobacterium were isolated from patients with cutaneous tuberculosis.and they were consistently identified as M.tuberculosis by biochemistry and molecular biology.Absolute concentration method was used to test the susceptibility of these isolates to isoniazid.streptomycin.rifampicin and ethambutol.For two strains resistant to streptomycin,PCR and sequencing were performed to analyse the mutation of rpsL gene.Results Out ofthe 16 strains of M tuberculosis.2 were resistant to streptomycin but sensitive to isoniazid,rifampicin and ethambutol,and 14 were sensitive to isoniazid,streptomycin,rifampicin and ethambutol.Sequencing of the rpsL gene revealed a mutation of AAG to AGG at codon 43 in one streptomycinresistant strain and a substitution of CGC bv CAC at codon 54 in another resistant strain.Conclusions In past five years,the resistance ratio of M tuberculosis was low in patients with cutaneous tuberculosis,and streptomycin resistance predominated in these strains.
