中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
14期
1875-1877
,共3页
鬼针草属%高效液相色谱%指纹图谱
鬼針草屬%高效液相色譜%指紋圖譜
귀침초속%고효액상색보%지문도보
Bidens%HPLC%Fingerprint
目的 对鬼针草药材高效液相色谱法(HPLC)指纹图谱进行研究,建立鬼针草药材的指纹图谱研究方法.方法 采用HPLC,ODS C18色谱柱(4.6 mm ×255 mm,25μm);乙腈-3%冰乙酸线性梯度洗脱;流速:1.0 ml/min;检测波长:363 nm.结果 建立了鬼针草药材的标准指纹图谱,并检测10批鬼针草药材与标准指纹图谱的相似度在0.941～0.999之间.结论 本实验所建立的方法具有准确可靠、重复性好、特征性强、方法简便等特点,可作为鬼针草药材的质量评价的重要依据之一.
目的 對鬼針草藥材高效液相色譜法(HPLC)指紋圖譜進行研究,建立鬼針草藥材的指紋圖譜研究方法.方法 採用HPLC,ODS C18色譜柱(4.6 mm ×255 mm,25μm);乙腈-3%冰乙痠線性梯度洗脫;流速:1.0 ml/min;檢測波長:363 nm.結果 建立瞭鬼針草藥材的標準指紋圖譜,併檢測10批鬼針草藥材與標準指紋圖譜的相似度在0.941～0.999之間.結論 本實驗所建立的方法具有準確可靠、重複性好、特徵性彊、方法簡便等特點,可作為鬼針草藥材的質量評價的重要依據之一.
목적 대귀침초약재고효액상색보법(HPLC)지문도보진행연구,건립귀침초약재적지문도보연구방법.방법 채용HPLC,ODS C18색보주(4.6 mm ×255 mm,25μm);을정-3%빙을산선성제도세탈;류속:1.0 ml/min;검측파장:363 nm.결과 건립료귀침초약재적표준지문도보,병검측10비귀침초약재여표준지문도보적상사도재0.941～0.999지간.결론 본실험소건립적방법구유준학가고、중복성호、특정성강、방법간편등특점,가작위귀침초약재적질량평개적중요의거지일.
Objective The fingerprint method based on high-performance liquid chromatography for the quality control of bidens bipinnata L were firstly described and optimized. Methods HPLC system was used to obtain the fingerprint spectrum of bidens bipinnata L. The chromatography condition was ODS C18 column (4. 6mm×255mm,25μm),mobile phase Methanol-0. 2% HAc,and flow speed 1. 0ml ? min-1, detection wavelength 363nm.Results In this experiment,the standard fingerprint had been established and the similitude degree of 10 batches bidens bipinnata L. has also been obtained(0.941～0.999). Conclusion The method was accurate,reliable,good reproducibility, strong characteristic and could be used for the standard of general judgement and the quality estimation about the bidens bipinnata L.
