中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
3期
221-223
,共3页
扁平苔癣%凋亡抑制蛋白质类%细胞凋亡
扁平苔癬%凋亡抑製蛋白質類%細胞凋亡
편평태선%조망억제단백질류%세포조망
Lichen planus%Apoptosis proteins inhibitor%Apeptesis
目的 探讨凋亡抑制蛋白c-FLIP(细胞型Fas相关死亡区域蛋白样IL-1β转换酶抑制蛋白)在扁平苔癣患者外周血和皮损中的表达和分布情况.方法 采用流式细胞仪检测30例扁平苔癣患者和20例正常人对照组外周血T细胞内c-FLIP表达阳性率,采用免疫组化方法 检测c-FLIP在其皮损中的表达.结果 扁平苔癣患者外周血T细胞c-FLIP表达(6.32%±1.17%)明显高于正常人对照组(2.28%±0.54%),P<0.05.而两组在外周血B细胞内的表达分别为0.78%±0.16%和0.69%±0.18%,差异无统计学意义(P>0.05).c-FLTP蛋白在扁平苔癣患者皮损中的表达(89.73%±5.24%)明显高于正常人对照组(121.58%±7.93%),P<0.01.结论 凋亡抑制蛋白c-FLIP在扁平苔癣患者的外周血T细胞和皮损内明显高表达,可能参与扁平苔癣患者T细胞的增殖.
目的 探討凋亡抑製蛋白c-FLIP(細胞型Fas相關死亡區域蛋白樣IL-1β轉換酶抑製蛋白)在扁平苔癬患者外週血和皮損中的錶達和分佈情況.方法 採用流式細胞儀檢測30例扁平苔癬患者和20例正常人對照組外週血T細胞內c-FLIP錶達暘性率,採用免疫組化方法 檢測c-FLIP在其皮損中的錶達.結果 扁平苔癬患者外週血T細胞c-FLIP錶達(6.32%±1.17%)明顯高于正常人對照組(2.28%±0.54%),P<0.05.而兩組在外週血B細胞內的錶達分彆為0.78%±0.16%和0.69%±0.18%,差異無統計學意義(P>0.05).c-FLTP蛋白在扁平苔癬患者皮損中的錶達(89.73%±5.24%)明顯高于正常人對照組(121.58%±7.93%),P<0.01.結論 凋亡抑製蛋白c-FLIP在扁平苔癬患者的外週血T細胞和皮損內明顯高錶達,可能參與扁平苔癬患者T細胞的增殖.
목적 탐토조망억제단백c-FLIP(세포형Fas상관사망구역단백양IL-1β전환매억제단백)재편평태선환자외주혈화피손중적표체화분포정황.방법 채용류식세포의검측30례편평태선환자화20례정상인대조조외주혈T세포내c-FLIP표체양성솔,채용면역조화방법 검측c-FLIP재기피손중적표체.결과 편평태선환자외주혈T세포c-FLIP표체(6.32%±1.17%)명현고우정상인대조조(2.28%±0.54%),P<0.05.이량조재외주혈B세포내적표체분별위0.78%±0.16%화0.69%±0.18%,차이무통계학의의(P>0.05).c-FLTP단백재편평태선환자피손중적표체(89.73%±5.24%)명현고우정상인대조조(121.58%±7.93%),P<0.01.결론 조망억제단백c-FLIP재편평태선환자적외주혈T세포화피손내명현고표체,가능삼여편평태선환자T세포적증식.
Objective To investigate the expression and distribution of cellular FLICE( Fas-assoelated death domain-like interleukin-I β-converting enzyme-like)-inhibitory protein (c-FLIP) in peripheral blood and lesions of lichen planus patients. Methods Peripheral blood and skin samples were obtained from 30 patients with lichen planns and 20 normal controls. Flow cytometry was used to detect intracellular c-Fl,lP in peripheral T and B lymphocytes, and immunohistochemistry was used to examine the expression of c-FLIP in lesionai tissue. Results Based on the pesitivity rate of c-FLIP, there was a significant increase in T iymphocytes in lichen planus compared with normal controls(6.32%±1. 17% vs 2.28%±0.54%, P < 0.05 ), while no significant difference was found in B lymphocytes between two groups (0.78% ±0. 16% vs 0.69% ± 0. 18%, P >0. 05 ). The expression intensity of c-FLIP in keratinocytes was also higher in lichen planusthanthatinnormalcontrols ( 89.73%± 5.24% vs 121.58% ±7.93% ,P < 0. 01 ). Conclusion c-FLIP is highly expressed in lesions and peripheral T lymphocytes of patients with lichen planus, which suggests the possible involvement of c-FLIP in the proliferation of T lymphocytes in lichen planns.
