CAJ | 학술논문

在我国,昆明小鼠作为一种实验动物广泛应用于药理和遗传学相关的研究领域.但由于昆明小鼠属于远交群,而且不同地区的种群间已经出现了严重分化,缺乏具有显著特征的近交系,这使得它在生物学上的应用受到了很大的限制.研究人员已经以昆明小鼠为背景培育出了几个可能的近交系,但由于缺乏可靠的遗传检测,至今未得到广泛的认可和应用.文章收集昆明小鼠的4个已经60代以上兄妹交配繁殖的可能近交系,并以两个标准近交系BALB/c和C57BL/6为参照,利用30个微卫星标记对每个品系的5只小鼠进行了微卫星基因分型,进而分析其遗传纯度.结果发现,品系A1和品系N4在本研究所用的30个位点均呈纯合状态;而T2和N2均在D15Mit16位点呈杂合状态.本研究第一次为我国昆明小鼠近交系的遗传学纯度提供了可靠的分子水平证据.今后应当加强昆明小鼠近交系的标准化,以扩大其在遗传学方面的应用.
재아국,곤명소서작위일충실험동물엄범응용우약리화유전학상관적연구영역.단유우곤명소서속우원교군,이차불동지구적충군간이경출현료엄중분화,결핍구유현저특정적근교계,저사득타재생물학상적응용수도료흔대적한제.연구인원이경이곤명소서위배경배육출료궤개가능적근교계,단유우결핍가고적유전검측,지금미득도엄범적인가화응용.문장수집곤명소서적4개이경60대이상형매교배번식적가능근교계,병이량개표준근교계BALB/c화C57BL/6위삼조,이용30개미위성표기대매개품계적5지소서진행료미위성기인분형,진이분석기유전순도.결과발현,품계A1화품계N4재본연구소용적30개위점균정순합상태;이T2화N2균재D15Mit16위점정잡합상태.본연구제일차위아국곤명소서근교계적유전학순도제공료가고적분자수평증거.금후응당가강곤명소서근교계적표준화,이확대기재유전학방면적응용.
Chinese Kun Ming (KM) mouse, an outbreed strain of laboratory animal, has been widely utilized in related pharmaceutical and genetic studies throughout China. However, the value of KM mice to the research community has been severely limited,partially due to the fact that well-characterized inbred strain of KM mice is not available. Several expected inbred strains from KM mice have been bred, but their genetic purity remains uncertain. In this study, four expected inbred strains of KM mice (A1, T2, N2,and N4) were chosen and their inbred degree were compared with two classical inbred mouse lines (BALB/c and C57BL/6) by analyzing the genotypes of about 30 microsatellite markers. In the four strains, A1 and N4 were homozygous at all genotyped loci, but N2 and T2 were only heterozygous at locus D15Mit16. These results indicate that the level of genetic purity/homozygousity of Al,N4, N2, and T2 inbred line is comparable to those of BALB/c and C57BL/6. This study provided the first and solid evidence for genetic purity of four expected inbred strains of KM mice. These 4 inbred mice strains should be well maintained for further characterization and utilization in genetic studies.