CAJ | 학술논문

采用毛细管电泳电导法对饲料添加剂氯化胆碱进行了测定.实验优化了缓冲介质、分离电压、毛细管长度和内径等参数.结果表明:在优化条件下,氯化胆碱在4 min左右可以实现分离检测,线性范围在41.8～1.20 μg/mL,检出限为0.8 μg/mL;迁移时间和峰面积的相对标准偏差分别为0.6%、2.1%;样品加标回收率为97%～105%.将该法用于粉剂和饲料中氯化胆碱的检测,取得了满意的结果.
채용모세관전영전도법대사료첨가제록화담감진행료측정.실험우화료완충개질、분리전압、모세관장도화내경등삼수.결과표명:재우화조건하,록화담감재4 min좌우가이실현분리검측,선성범위재41.8～1.20 μg/mL,검출한위0.8 μg/mL;천이시간화봉면적적상대표준편차분별위0.6%、2.1%;양품가표회수솔위97%～105%.장해법용우분제화사료중록화담감적검측,취득료만의적결과.
Determination of choline chloride in feedstuff by capillary electrophoresis with conductometric electrochemical detection was carried out. The effects of some important factors, such as the running buffer, the separation voltage and the length and inner diameter of capillary on separation were investigated. Under the optimum conditions, choline chloride was separated and detected in 4 min with a detection limit as low as 0.8 μg/mL.The response was linear in the range of 41.8～1.20 μg/mL. The relative standard deviations (n=8) of the migration time and peak area were 0.6% and 2.1%, respectively, and the recoveries for the spiked sample were in the range of 97%～105%. The method had high sensitivity and good reproducibility, and was applied to the determination of choline chloride in the powder sample and the feedstuff sample with satisfactory results.