西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2009年
6期
709-711
,共3页
张桂英%赵海军%信振江%曲景乐%王宝贵%王昊
張桂英%趙海軍%信振江%麯景樂%王寶貴%王昊
장계영%조해군%신진강%곡경악%왕보귀%왕호
硝酸亚铈%细胞周期%细胞培养
硝痠亞鈰%細胞週期%細胞培養
초산아시%세포주기%세포배양
cerium nitrate%cell cycle%cellular culture
目的 探讨硝酸亚铈体外毒性、抑瘤率及其对细胞周期的影响.方法 采用MTT法检测硝酸亚铈对正常细胞系FL、L929体外毒性,对肿瘤细胞系Hela、SGC-7901、B16、Lewis、K562、H_(22)的体外抑瘤效应.采用流式细胞仪检测硝酸亚铈对SGC-7901的细胞周期影响.结果 硝酸亚铈在0.64 mmol/L以下时对FL、L929无任何毒副作用;硝酸亚铈在0.64 mmol/L以上时对以上6种肿瘤细胞均有明显的抑制增殖作用,在0.08 mmol/L时对HeLa、SGC-7901、B16及K562肿瘤细胞表现出显著的抑制增殖作用,在0.01、0.04 mmol/L时,对H_(22)亦有显著抑制作用;0.08 mmol/L硝酸亚铈作用48 h,能显著延长SGC-7901细胞株的G0/G1期,缩短S期;1.28 mmol/L硝酸亚铈作用48 h,除了能够显著延长SGC-7901细胞株的G0/G1期,显著缩短S期以外,还能延长G2/M期.结论 硝酸亚铈对正常细胞具有低毒性和高度选择性,在体外通过影响细胞周期来抑制多种肿瘤细胞的生长和增殖.
目的 探討硝痠亞鈰體外毒性、抑瘤率及其對細胞週期的影響.方法 採用MTT法檢測硝痠亞鈰對正常細胞繫FL、L929體外毒性,對腫瘤細胞繫Hela、SGC-7901、B16、Lewis、K562、H_(22)的體外抑瘤效應.採用流式細胞儀檢測硝痠亞鈰對SGC-7901的細胞週期影響.結果 硝痠亞鈰在0.64 mmol/L以下時對FL、L929無任何毒副作用;硝痠亞鈰在0.64 mmol/L以上時對以上6種腫瘤細胞均有明顯的抑製增殖作用,在0.08 mmol/L時對HeLa、SGC-7901、B16及K562腫瘤細胞錶現齣顯著的抑製增殖作用,在0.01、0.04 mmol/L時,對H_(22)亦有顯著抑製作用;0.08 mmol/L硝痠亞鈰作用48 h,能顯著延長SGC-7901細胞株的G0/G1期,縮短S期;1.28 mmol/L硝痠亞鈰作用48 h,除瞭能夠顯著延長SGC-7901細胞株的G0/G1期,顯著縮短S期以外,還能延長G2/M期.結論 硝痠亞鈰對正常細胞具有低毒性和高度選擇性,在體外通過影響細胞週期來抑製多種腫瘤細胞的生長和增殖.
목적 탐토초산아시체외독성、억류솔급기대세포주기적영향.방법 채용MTT법검측초산아시대정상세포계FL、L929체외독성,대종류세포계Hela、SGC-7901、B16、Lewis、K562、H_(22)적체외억류효응.채용류식세포의검측초산아시대SGC-7901적세포주기영향.결과 초산아시재0.64 mmol/L이하시대FL、L929무임하독부작용;초산아시재0.64 mmol/L이상시대이상6충종류세포균유명현적억제증식작용,재0.08 mmol/L시대HeLa、SGC-7901、B16급K562종류세포표현출현저적억제증식작용,재0.01、0.04 mmol/L시,대H_(22)역유현저억제작용;0.08 mmol/L초산아시작용48 h,능현저연장SGC-7901세포주적G0/G1기,축단S기;1.28 mmol/L초산아시작용48 h,제료능구현저연장SGC-7901세포주적G0/G1기,현저축단S기이외,환능연장G2/M기.결론 초산아시대정상세포구유저독성화고도선택성,재체외통과영향세포주기래억제다충종류세포적생장화증식.
Objective To investigate the cytotoxicity on normal cell lines of cerium nitrate, the inhibitory effect on tumor cell lines and the effect on cell cycle in vitro. Methods The cytotoxicity of cerium nitrate on FL, L929 and inhibition rate on Hela, SGC-7901, B16, Lewis, K562, and H_(22) in vitro were detected with the method of MTT. The effect of cerium nitrate on cell cycle of SGC-7901 was studied by one-fluorescence dying method on flow cytometer. Results Cerium nitrate did not show the toxic activities on FL and L929 cell lines when the dose of cerium nitrate was it could noticeably inhibit the growth and proliferation of tumor cells nitrate inhibited the growth and proliferation of tumer cells including HeLa, SGC-7901, B16 and K562; cerium nitrate had a noticeably inhibitory effect on the growth and proliferation of H_(22) cell lines in vitro on Conclusion Cerium nitrate has the property of low toxicity and high selectivity on normal cells, but it can inhibit growth and proliferation of many kinds of tumor cell lines by adjusting cell cycle.
