CAJ | 학술논문

以无单唾液酸四己糖神经节苷脂(GM1)的神经节苷脂为唯一C源,从土壤中筛选出1株能以多唾液酸神经节苷脂为底物,转化生成GM1的产唾液酸酶微生物,经鉴定为溶黄嘌呤厄菌(Oerskovia xanthineolytica).利用单因素法和响应面分析法对溶黄嘌呤厄菌产生唾液酸酶的条件进行优化,酶活提高了4.89倍.利用优化后培养条件,以神经节苷脂混合体为底物,经过微生物生物转化后,单唾液酸神经节苷脂GM1的含量从10%提高到83.7%.
이무단타액산사기당신경절감지(GM1)적신경절감지위유일C원,종토양중사선출1주능이다타액산신경절감지위저물,전화생성GM1적산타액산매미생물,경감정위용황표령액균(Oerskovia xanthineolytica).이용단인소법화향응면분석법대용황표령액균산생타액산매적조건진행우화,매활제고료4.89배.이용우화후배양조건,이신경절감지혼합체위저물,경과미생물생물전화후,단타액산신경절감지GM1적함량종10%제고도83.7%.
Oerskovia xanthineolytica-the strain which used to convert polysialoganglioside into monosialo-tetrahexosyl ganglioside(GM1) was efficiently screened from soil through high flux screening of sialidase-producing bacterium with free GM1 gangliosides as the sole source carbon.The concentration for maximum enzyme activity was optimized by single factor response surface analysis.The strain was cultured in the optimized medium containing crude gangliosides(10% w/v) at 37℃ for 12 h.Most of the polysialo-gangliosides are converted into GMl.The relative content of GM1 increased from 10% to 83.7% in the crude gangliosides.