CAJ | 학술논문

以中熟红富士品种红将军的继代培养4～5代试管苗为试材,进行脱毒处理,并利用ELISA和RT-PCR技术检测脱毒效果.结果表明:红将军苹果试管苗在38℃高温条件下恒定热处理20 d,再二次茎尖培养后,试管苗脱毒率达86.4%～100%,可以有效脱除ASSVd、ASGV、ASPV、ApMV和ACLSV等主要的苹果病毒.
이중숙홍부사품충홍장군적계대배양4～5대시관묘위시재,진행탈독처리,병이용ELISA화RT-PCR기술검측탈독효과.결과표명:홍장군평과시관묘재38℃고온조건하항정열처리20 d,재이차경첨배양후,시관묘탈독솔체86.4%～100%,가이유효탈제ASSVd、ASGV、ASPV、ApMV화ACLSV등주요적평과병독.
In vitro seedlings subeultured 4 -5 generation of midseason Fuji apple variety Red General were eliminated viruses,and then were detected by ELISA and RT-PCR. Results showed that keeping constant heat treatment conditions at 38℃ for 20 days,the virus elimination rate of Red General apple reached 86.4% -100%, and major apple viruses such as ASSVd,ASGV,ASPV,ApMV and ACLSV were eluted well.