CAJ | 학술논문

目的:建立以聚酰胺薄膜为固定相,微乳液为展开剂,同时分离鉴别虎杖中白藜芦醇、虎杖苷顺反异构体的方法.方法:以SDS-正丁醇-正庚烷-水微乳液为展开剂,并配以甲酸、甲醇为有机改性剂,考察了微乳液对分离度的影响,通过调节展开剂的组成,寻找出最佳的薄层分离条件并分离检测了6个产地的虎杖药材.结果:微乳液-甲酸-甲醇(1.0:2.0:1.0)为适宜的展开剂,其中微乳液含SDS 4%、含水量80%.与硅胶薄层比较,分离效果明显提高.结论:建立了一种分离鉴别虎杖中自藜芦醇和虎杖苷顺、反异构体的方法.方法简便、快速和准确.
목적:건립이취선알박막위고정상,미유액위전개제,동시분리감별호장중백려호순、호장감순반이구체적방법.방법:이SDS-정정순-정경완-수미유액위전개제,병배이갑산、갑순위유궤개성제,고찰료미유액대분리도적영향,통과조절전개제적조성,심조출최가적박층분리조건병분리검측료6개산지적호장약재.결과:미유액-갑산-갑순(1.0:2.0:1.0)위괄의적전개제,기중미유액함SDS 4%、함수량80%.여규효박층비교,분리효과명현제고.결론:건립료일충분리감별호장중자려호순화호장감순、반이구체적방법.방법간편、쾌속화준학.
AIM:To investigate the application of microemulsion TLC to separation and identification of(Z)and(E)-resveratrol and piceid in Polygonum cuspidatum based on polyamide film as stationary phase.METHODS:SDS-n-butane-n-heptane-aqueous microemulsion as the development in combination with aminic acid and methonal as the modifier,the influence of the change in components of microemulsion on the resolution was exam-ined to search for the optimal separation condition of Polygonm cuspidatum extract collected from 6 production places.RESULTS:The best mobile phase was the microemulsion containing 4%SDS,80%water-methyl acidmethanol(1.0:2.0:1.0).As compared with conventional TLC,resolution of microemulsion TLC for resveratrol and piceid were improved markedly.CONCLUSION:Microemulsion TLC is an efficient,simple and new method for separating and identifing(Z)-and(E)-diastereomers of resveratrol and piceid in Polygonum cuspidatum.