CAJ | 학술논문

目的:探讨CTGF对糖尿病大鼠视网膜内细胞凋亡的影响.方法:将60大鼠分为对照组,糖尿病4,8,12,16wk组和干预组.糖尿病大鼠应用STZ腹腔注射诱导成模.干预组大鼠于糖尿病成模后16wk玻璃体腔注射CTGFsiRNA来造成CTGF基因的沉默.取各组视网膜组织,应用RT-PCR检测视网膜内的CTGF基因表达,Tunnel法检测视网膜凋亡细胞的情况.结果:糖尿病组视网膜内CTGF表达和凋亡细胞数较正常组明显增加.凋亡发生在建模后4wk,并随着时间的延长而加重,在24wk时,凋亡细胞数为25.8个/mm2.CTGF表达于8wk时出现升高,到16wk时升高更明显.CTGFsiRNA处理后凋亡细胞数明显降低.视网膜内CTGF和细胞凋亡之间具有明显的相关性.结论:CTGF参与了糖尿病视网膜早期病变的细胞凋亡机制,CTGFsiRNA有利于改善糖尿病早期视网膜由于凋亡所致的细胞丢失.
목적:탐토CTGF대당뇨병대서시망막내세포조망적영향.방법:장60대서분위대조조,당뇨병4,8,12,16wk조화간예조.당뇨병대서응용STZ복강주사유도성모.간예조대서우당뇨병성모후16wk파리체강주사CTGFsiRNA래조성CTGF기인적침묵.취각조시망막조직,응용RT-PCR검측시망막내적CTGF기인표체,Tunnel법검측시망막조망세포적정황.결과:당뇨병조시망막내CTGF표체화조망세포수교정상조명현증가.조망발생재건모후4wk,병수착시간적연장이가중,재24wk시,조망세포수위25.8개/mm2.CTGF표체우8wk시출현승고,도16wk시승고경명현.CTGFsiRNA처리후조망세포수명현강저.시망막내CTGF화세포조망지간구유명현적상관성.결론:CTGF삼여료당뇨병시망막조기병변적세포조망궤제,CTGFsiRNA유리우개선당뇨병조기시망막유우조망소치적세포주실.
·AIM: To detect the effect of CTGF on the apoptosis in the diabetic retina with small interfering RNAs (siRNA) targeting with CTGF. ·METHODS: A total of 60 rats were divided into six groups including control group, diabetic 4,8,12,16 weeks group, and interference group. Diabetic rats were induced by STZ intra-peritoneal. At 4, 8, 12, 16 weeks after diabetic setting up, retinas were obtained from control, diabetic rats and diabetic animals treated by intravitreal injection of CTGFsiRNA to suppress the expression of CTGF mRNA. Retinal cells apoptosis was detected by Tunnel staining and mRNA expression of CTGF was analyzed by RT-PCR.·RESULTS: The levels of CTGF and the apoptosis in the retinas of diabetic rats were significantly higher than those in the controls. Apoptosis occurred at 4 weeks after a diabetic model setting up, became serious with the diabetes developing, while CTGF elevated at 8 weeks. The cell apoptosis counts increased to 25.8cells/mm2 at 24 weeks of diabetes. SiRNA-mediated inhibition of CTGF mRNA resulted in a significant decrease in apoptosis. Significant correlations were found between CTGF and apoptosis in the retina.·CONCLUSION: These results suggest that CTGF might be involved in retinal cells apoptosis which is a characteristic of early diabetic retina. siRNA targeting CTGF seems to have the advantage of ameliorating retinal cells lost.