CAJ | 학술논문

目的探索利用50％氰氨化钙(商品名为荣宝)在洲滩易感地带杀灭血吸虫虫卵和尾蚴的简易方法,继而达到控制传染源的目的.方法收集含血吸虫虫卵的阳性的新鲜牛粪.在夏、秋、冬3个季节,分别按照每100 g阳性牛粪加荣宝3 g和5 g的比例,在易感地带以搅拌、表面喷撒和粪内中置等3组方式施药,并设不施药阳性牛粪对照组,于不同时段分别取样,用塑料杯顶管孵化法检查毛蚴.用浓度为10 g/m3的荣宝对血吸虫易感水体进行血吸虫尾蚴杀灭,在施药前及施药后不同时段用哨鼠测定血吸虫易感水体.结果夏季,搅拌组和表面喷撒组施药后第2天均未发现毛蚴,粪内中置组第3天未发现毛蚴；秋季,施药各组第2天取样均可发现毛蚴1～5条,第3天除粪内中置组仍然发现毛蚴1～5条外,其余施药各组未发现毛蚴；冬季,施药各组第3天取样孵化,除搅拌组外均可发现毛蚴2～3条,第7天抽样孵化未发现毛蚴；夏、秋、冬3季对照组第7天仍然有6 ～ 10条毛蚴.用荣宝处置血吸虫易感水体,处置前哨鼠血吸虫感染率为11.11％,处置后第1、15和30天,经解剖检查,哨鼠均为阴性,亦未发现死鱼.结论 3组施药方式在易感地带均具有杀灭血吸虫虫卵的作用.其中,以每100 g牛粪加3 g荣宝的比例,采取表面喷撒的方法适宜现场应用,夏季效果更佳.采用荣宝10 g/m3的比例喷洒于现场血吸虫易感水体,可有效杀灭血吸虫尾蚴.2种方法同时使用,可达到净化血吸虫易感地带的目的.
목적 탐색이용50％청안화개(상품명위영보)재주탄역감지대살멸혈흡충충란화미유적간역방법,계이체도공제전염원적목적.방법 수집함혈흡충충란적양성적신선우분.재하、추、동3개계절,분별안조매100 g양성우분가영보3 g화5 g적비례,재역감지대이교반、표면분살화분내중치등3조방식시약,병설불시약양성우분대조조,우불동시단분별취양,용소료배정관부화법검사모유.용농도위10 g/m3적영보대혈흡충역감수체진행혈흡충미유살멸,재시약전급시약후불동시단용초서측정혈흡충역감수체.결과 하계,교반조화표면분살조시약후제2천균미발현모유,분내중치조제3천미발현모유；추계,시약각조제2천취양균가발현모유1～5조,제3천제분내중치조잉연발현모유1～5조외,기여시약각조미발현모유；동계,시약각조제3천취양부화,제교반조외균가발현모유2～3조,제7천추양부화미발현모유；하、추、동3계대조조제7천잉연유6 ～ 10조모유.용영보처치혈흡충역감수체,처치전초서혈흡충감염솔위11.11％,처치후제1、15화30천,경해부검사,초서균위음성,역미발현사어.결론 3조시약방식재역감지대균구유살멸혈흡충충란적작용.기중,이매100 g우분가3 g영보적비례,채취표면분살적방법괄의현장응용,하계효과경가.채용영보10 g/m3적비례분쇄우현장혈흡충역감수체,가유효살멸혈흡충미유.2충방법동시사용,가체도정화혈흡충역감지대적목적.
Objective To explore a rapid and easy measure of perishing Schistosoma ova and cercariae by 50％ calcium cyanamide (commercial name Rongbao) at hot spots in Lake Regions,so as to meet the purpose of infection sources control.Methods The fresh positive cattle excrement containing schistosoma ova was collected for experientment.In summer,autumn and winter,cattle excrement was applied by the measures of stiring (SG),surface spraying (SSG) and middle-inside depositing (MDG) with the proportion at 3 g and/or 5 g of Rongbao per 100 g of cattle excrement at hot spots,the control group (CG) was cattle excrement with schistosoma ova without application.After applying Rongbao,the excrement was collected at different times and miracidia were examined by hatching method.For perishing Schistosoma cercaria in infectious water,the proportion of 10 g/m3 Rongbao were sprayed.The sentinel mice surveillance was implemented for infectious water detection before and after spraying.Results In summer,miracidia were not existed after 2 d in SG and SSG,and it was not existed after 3 d in MDG.In autumn,1-5 miracidia were existed after 2 d among 3 groups,and there were no miracidia in SG and SSG after 3 d,except 1-5 micacidia in MDG.In winter,2-3 miracidia were existed after 3 d except SG,no miracidia after 7 d in 3 groups.However,6-10 miracidia were existed in the control group after 7 d in summer,autumn and winter.As to the treatment of infectious water by Rongbao,the infection rate of sentinel mice was 11.11％ before treatment,while there were no infection at 1 d,15 d and 30 d after treatment examined by dissection,meanwhile,died fishes were not founded.Conclusion The 3 kinds of measures all have perishing effect on Schistosoma ova.According to the result for the field use,the suitable proportion and method are 3 g Rongbao per 100 g cattle excrement by SSG in summer.Schistosoma cercaria were perished by spraying Rongbao at the proportion of 10 g/m3 into infectious water.Above of all,the infection source of Schistosoma will be perished when SSG and infectious water spray are implemented at hot spots.