中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
6期
481-483
,共3页
抑郁症%海马%脑源性神经营养因子%探索行为
抑鬱癥%海馬%腦源性神經營養因子%探索行為
억욱증%해마%뇌원성신경영양인자%탐색행위
Depression%Hippocampus%Brain-derived neurotrophic factor%Exploratory behaviors
目的 研究睡眠剥夺对抑郁样模型大鼠探索行为的影响,并从脑源性神经营养因子(BDNF)的角度探讨其机制.方法 30只SD大鼠随机分为正常组、抑郁组和剥夺组,慢性不可预知性刺激法制备抑郁样模型；在第6,12,18,24天运用多平台水环境法对剥夺组进行连续24h的全睡眠剥夺,剥夺干预后随即对各组大鼠进行敞箱实验；免疫组织化学和逆转录聚合链式反应法测海马神经元BDNF表达水平.结果 第6,12,18,24天四次行为测试得分显示,剥夺组大鼠的探索行为得分呈现先降低后升高趋势,第24天探索行为得分与抑郁组比较差异有统计学意义(P＜0.01),而与正常组比较差异无统计学意义(P＞0.05).免疫组化结果显示,与抑郁组[积分光密度:108.53±4.45,阳性面积比:0.0747±0.0262]相比,剥夺组[积分光密度:116.00 ±5.61,阳性面积比:0.2034±0.0352]大鼠海马神经元BDNF蛋白表达明显增高(P＜0.05),而与正常组[积分光密度:117.27±10.66,阳性面积比:0.2252±0.1143]相比差异无统计学意义(P＞0.05).RT-PCR结果和免疫组化结果相符.结论 睡眠剥夺能改善抑郁模型大鼠的探索行为能力,其机制可能与提高海马组织BDNF的含量有关.
目的 研究睡眠剝奪對抑鬱樣模型大鼠探索行為的影響,併從腦源性神經營養因子(BDNF)的角度探討其機製.方法 30隻SD大鼠隨機分為正常組、抑鬱組和剝奪組,慢性不可預知性刺激法製備抑鬱樣模型；在第6,12,18,24天運用多平檯水環境法對剝奪組進行連續24h的全睡眠剝奪,剝奪榦預後隨即對各組大鼠進行敞箱實驗；免疫組織化學和逆轉錄聚閤鏈式反應法測海馬神經元BDNF錶達水平.結果 第6,12,18,24天四次行為測試得分顯示,剝奪組大鼠的探索行為得分呈現先降低後升高趨勢,第24天探索行為得分與抑鬱組比較差異有統計學意義(P＜0.01),而與正常組比較差異無統計學意義(P＞0.05).免疫組化結果顯示,與抑鬱組[積分光密度:108.53±4.45,暘性麵積比:0.0747±0.0262]相比,剝奪組[積分光密度:116.00 ±5.61,暘性麵積比:0.2034±0.0352]大鼠海馬神經元BDNF蛋白錶達明顯增高(P＜0.05),而與正常組[積分光密度:117.27±10.66,暘性麵積比:0.2252±0.1143]相比差異無統計學意義(P＞0.05).RT-PCR結果和免疫組化結果相符.結論 睡眠剝奪能改善抑鬱模型大鼠的探索行為能力,其機製可能與提高海馬組織BDNF的含量有關.
목적 연구수면박탈대억욱양모형대서탐색행위적영향,병종뇌원성신경영양인자(BDNF)적각도탐토기궤제.방법 30지SD대서수궤분위정상조、억욱조화박탈조,만성불가예지성자격법제비억욱양모형；재제6,12,18,24천운용다평태수배경법대박탈조진행련속24h적전수면박탈,박탈간예후수즉대각조대서진행창상실험；면역조직화학화역전록취합련식반응법측해마신경원BDNF표체수평.결과 제6,12,18,24천사차행위측시득분현시,박탈조대서적탐색행위득분정현선강저후승고추세,제24천탐색행위득분여억욱조비교차이유통계학의의(P＜0.01),이여정상조비교차이무통계학의의(P＞0.05).면역조화결과현시,여억욱조[적분광밀도:108.53±4.45,양성면적비:0.0747±0.0262]상비,박탈조[적분광밀도:116.00 ±5.61,양성면적비:0.2034±0.0352]대서해마신경원BDNF단백표체명현증고(P＜0.05),이여정상조[적분광밀도:117.27±10.66,양성면적비:0.2252±0.1143]상비차이무통계학의의(P＞0.05).RT-PCR결과화면역조화결과상부.결론 수면박탈능개선억욱모형대서적탐색행위능력,기궤제가능여제고해마조직BDNF적함량유관.
Objective To investigate the effects of exploratory behaviors on depression-like rats treated with sleep deprivation,and the mechanism of the point of brain-derived neurotrophic factor (BDNF).Methods 30 Sprague-Dawley (SD) rats were randomly divided into normal group,depression group and deprivation group,and the depression model of rats were established by chronic unpredictable stress.At the day of 6th,12th,18th,24th the deprivation group was deprived sleep for 24h by the method of multi-platform water environment,and then all groups were on the open-field test.The approach of immunohistochemistry and retrovirus polymerization chain reaction measured BDNF expression level in the hippocampal neurons.Results The four open-field test scores of the day of 6th,12th,18th,24th indicated that the scores of deprivation group was a low-to high trend,and at the open-field test of the 24th day was significantly different compared with depression group(P ＜0.01 ),while no significant difference was found compared with the normal group (P ＞ 0.05 ).Compared with depression group( gray level:108.53 ± 4.45,positive area rate:0.0747 ± 0.0262 ),BDNF protein expression was significantly higher in the hippocampal neurons of deprivation group( gray level:116.00 ±5.61,positive area rate:0.2034 ± 0.0352 ) (P ＜ 0.05 ),while there was no significant difference in normal group ( gray level:117.27 ±10.66,positive area rate:0.2252 ±0.1143 ) (P＞0.05 ).Results of RT-PCR were in accord with that of immunohistochemistry.Conclusion Sleep deprivation can improve the exploratory behaviors of depressive rats and the mechanism may be related to the increasing concentration of BDNF in hippocampal neurons.
