CAJ | 학술논문

目的观察肠淋巴液引流对创伤失血性休克(T/HS)大鼠多器官损伤的影响,探讨其作用机制.方法 Wistar雄性大鼠分为对照组、淋巴液引流组与淋巴液回流组,后两组复制T/HS模型,淋巴液引流组行肠淋巴液引流.观察各组大鼠平均动脉血压(MAP),检测反映酸碱状态以及肝、肾、心肌的生化指标,对肺、肝、肾、心肌进行病理观察并检测其ATP含量和ATPase活性的变化.结果淋巴液引流组在输液80 min后多个时相点的MAP、多个组织器官的ATP含量、ATPase活性均高于淋巴液回流组,淋巴液引流组大鼠的多项生化指标优于淋巴液回流组,差异有统计学意义;淋巴液回流组可见炎症、淤血、变性、坏死等变化,而淋巴液引流组病变轻微.结论休克淋巴液引流可减轻T/HS大鼠的多器官损伤,其机制与改善能量代谢、维持血压和酸碱状态有关.
목적 관찰장림파액인류대창상실혈성휴극(T/HS)대서다기관손상적영향,탐토기작용궤제.방법 Wistar웅성대서분위대조조、림파액인류조여림파액회류조,후량조복제T/HS모형,림파액인류조행장림파액인류.관찰각조대서평균동맥혈압(MAP),검측반영산감상태이급간、신、심기적생화지표,대폐、간、신、심기진행병리관찰병검측기ATP함량화ATPase활성적변화.결과 림파액인류조재수액80 min후다개시상점적MAP、다개조직기관적ATP함량、ATPase활성균고우림파액회류조,림파액인류조대서적다항생화지표우우림파액회류조,차이유통계학의의;림파액회류조가견염증、어혈、변성、배사등변화,이림파액인류조병변경미.결론 휴극림파액인류가감경T/HS대서적다기관손상,기궤제여개선능량대사、유지혈압화산감상태유관.
Objective To observe the effect of shock lymph drainage on multiple organ injury of rats with traumatic hemorrhagic shock (THS) and discuss the relating mechanism. Methods Male Wistar rats were divided into control group, lymph drainage group and lymph return group. The THS model was established in lymph drainage group and lymph return group, when the shock mesenteric lymph was drained in lymph drainage group. The change of the mean arterial pressure ( MAP), the biochemical indices of liver, kidney, myocardium and acid-base, the morphology, ATP contents and ATPase activities of lung, kidney, liver and myocardium were observed. Results The MAP at multiple time points after 80 minutes of infusion, the ATP contents and ATPase activities of multiple organs in lymph drainage group were higher than those in lymph return group. Multiple biochemical indices in lymph drainage group were superior to those in lymph return group, with statistical difference. The inflammation, congestion, degeneration and necrosis were found in organs of lymph return group, but only mild lesions could be seen in lymph drainage group. Conclusions The shock lymph drainage can alleviate multiple organ injury of THS rats, mechanism of which is correlated with improvement of the energy metabolism and maintenance of MAP and acid-base status.