中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2011年
1期
54-58
,共5页
李毅%王洪瑾%宋学芳%杨惠莲
李毅%王洪瑾%宋學芳%楊惠蓮
리의%왕홍근%송학방%양혜련
烧伤%硫化氢%内脏%生化指标%胱硫醚-γ-裂合酶
燒傷%硫化氫%內髒%生化指標%胱硫醚-γ-裂閤酶
소상%류화경%내장%생화지표%광류미-γ-렬합매
Burns%Hydrogen sulfide%Viscera%Biochemical index%Cystathionine gammalyase
目的 观察硫化氢及其所生成的胱硫醚-γ-裂合酶(CSE)在大鼠严重烧伤后的变化规律,分析其对严重烧伤大鼠重要脏器的影响.方法 健康雄性SD大鼠104只,按照随机数字表法分为正常对照组8只、烧伤组48只、硫化氢干预组48只.硫化氢干预组大鼠于伤前5 d起腹腔注射硫氢化钠(56 μmol/kg,每日定时1次),注射5 d后与烧伤组大鼠均造成30%TBSAⅢ度烧伤.于伤后2、6、12、24、48、96 h(每时相点8只)检测烧伤组大鼠血清硫化氢含量,采集心、肝、肾、肺、胃组织标本检测CSE转化率;检测烧伤组和硫化氢干预组大鼠上述各时相点血清ALT、AST、心肌型肌酸激酶同工酶(CK-MB)、尿素氮、肌酐含量,观察上述脏器组织形态学变化.正常对照组检测指标同上.对数据行SNK-q检验、t检验、Pearson相关分析(血清硫化氢含量与CSE转化率、血生化指标).结果 烧伤组大鼠伤后各时相点血清硫化氢含量和各脏器组织CSE转化率普遍低于正常对照组,伤后6或12 h达到最低点.与正常对照组相比,烧伤组伤后2 h血清ALT、AST、CK-MB、尿素氮、肌酐含量明显升高,伤后6 h尿素氮[(32.5±9.8)mmol/L]和肌酐[(65±9)μmol/L]达峰值,伤后12 h ALT[(423±59)U/L]、AST[(993±60)U/L]及CK-MB[(49 261±6637)U/L]达峰值,伤后48 h上述指标仍高于正常对照组(t=3.157～8.386,P值均小于0.05).硫化氢干预组大鼠伤后各时相点血清ALT、AST、CK-MB、尿素氮、肌酐含量变化趋势与烧伤组大致相同,普遍低于烧伤组.组织形态学观察显示,伤后24 h烧伤组大鼠心、肝、肺、肾和胃组织损害严重,硫化氢干预组损伤较之明显减轻.烧伤组血清硫化氢含量与各脏器组织CSE转化率、各生化指标呈显著正相关(r值分别为0.639～0.894、0.301～0.585,P＜0.005或P＜0.001).结论 硫化氢/CSE体系可能参与了大鼠烧伤后的病理生理过程.补充外源性硫化氢对严重烧伤大鼠的重要脏器可产生保护作用.
目的 觀察硫化氫及其所生成的胱硫醚-γ-裂閤酶(CSE)在大鼠嚴重燒傷後的變化規律,分析其對嚴重燒傷大鼠重要髒器的影響.方法 健康雄性SD大鼠104隻,按照隨機數字錶法分為正常對照組8隻、燒傷組48隻、硫化氫榦預組48隻.硫化氫榦預組大鼠于傷前5 d起腹腔註射硫氫化鈉(56 μmol/kg,每日定時1次),註射5 d後與燒傷組大鼠均造成30%TBSAⅢ度燒傷.于傷後2、6、12、24、48、96 h(每時相點8隻)檢測燒傷組大鼠血清硫化氫含量,採集心、肝、腎、肺、胃組織標本檢測CSE轉化率;檢測燒傷組和硫化氫榦預組大鼠上述各時相點血清ALT、AST、心肌型肌痠激酶同工酶(CK-MB)、尿素氮、肌酐含量,觀察上述髒器組織形態學變化.正常對照組檢測指標同上.對數據行SNK-q檢驗、t檢驗、Pearson相關分析(血清硫化氫含量與CSE轉化率、血生化指標).結果 燒傷組大鼠傷後各時相點血清硫化氫含量和各髒器組織CSE轉化率普遍低于正常對照組,傷後6或12 h達到最低點.與正常對照組相比,燒傷組傷後2 h血清ALT、AST、CK-MB、尿素氮、肌酐含量明顯升高,傷後6 h尿素氮[(32.5±9.8)mmol/L]和肌酐[(65±9)μmol/L]達峰值,傷後12 h ALT[(423±59)U/L]、AST[(993±60)U/L]及CK-MB[(49 261±6637)U/L]達峰值,傷後48 h上述指標仍高于正常對照組(t=3.157～8.386,P值均小于0.05).硫化氫榦預組大鼠傷後各時相點血清ALT、AST、CK-MB、尿素氮、肌酐含量變化趨勢與燒傷組大緻相同,普遍低于燒傷組.組織形態學觀察顯示,傷後24 h燒傷組大鼠心、肝、肺、腎和胃組織損害嚴重,硫化氫榦預組損傷較之明顯減輕.燒傷組血清硫化氫含量與各髒器組織CSE轉化率、各生化指標呈顯著正相關(r值分彆為0.639～0.894、0.301～0.585,P＜0.005或P＜0.001).結論 硫化氫/CSE體繫可能參與瞭大鼠燒傷後的病理生理過程.補充外源性硫化氫對嚴重燒傷大鼠的重要髒器可產生保護作用.
목적 관찰류화경급기소생성적광류미-γ-렬합매(CSE)재대서엄중소상후적변화규률,분석기대엄중소상대서중요장기적영향.방법 건강웅성SD대서104지,안조수궤수자표법분위정상대조조8지、소상조48지、류화경간예조48지.류화경간예조대서우상전5 d기복강주사류경화납(56 μmol/kg,매일정시1차),주사5 d후여소상조대서균조성30%TBSAⅢ도소상.우상후2、6、12、24、48、96 h(매시상점8지)검측소상조대서혈청류화경함량,채집심、간、신、폐、위조직표본검측CSE전화솔;검측소상조화류화경간예조대서상술각시상점혈청ALT、AST、심기형기산격매동공매(CK-MB)、뇨소담、기항함량,관찰상술장기조직형태학변화.정상대조조검측지표동상.대수거행SNK-q검험、t검험、Pearson상관분석(혈청류화경함량여CSE전화솔、혈생화지표).결과 소상조대서상후각시상점혈청류화경함량화각장기조직CSE전화솔보편저우정상대조조,상후6혹12 h체도최저점.여정상대조조상비,소상조상후2 h혈청ALT、AST、CK-MB、뇨소담、기항함량명현승고,상후6 h뇨소담[(32.5±9.8)mmol/L]화기항[(65±9)μmol/L]체봉치,상후12 h ALT[(423±59)U/L]、AST[(993±60)U/L]급CK-MB[(49 261±6637)U/L]체봉치,상후48 h상술지표잉고우정상대조조(t=3.157～8.386,P치균소우0.05).류화경간예조대서상후각시상점혈청ALT、AST、CK-MB、뇨소담、기항함량변화추세여소상조대치상동,보편저우소상조.조직형태학관찰현시,상후24 h소상조대서심、간、폐、신화위조직손해엄중,류화경간예조손상교지명현감경.소상조혈청류화경함량여각장기조직CSE전화솔、각생화지표정현저정상관(r치분별위0.639～0.894、0.301～0.585,P＜0.005혹P＜0.001).결론 류화경/CSE체계가능삼여료대서소상후적병리생리과정.보충외원성류화경대엄중소상대서적중요장기가산생보호작용.
Objective To investigate the changes in hydrogen sulfide (H2S) and cystathionine gamma-lyase (CSE) in rats with severe burn, and to analyze the effects on important organs. Methods One hundred and four healthy male SD rats were divided into normal control group(NC, n = 8), burn group (B, n =48), and H2S intervention group (HI, n =48) according to the random number table. SD rats in HI group were intraperitoneally injected with NaHS (56 μmol/kg) once a day for 5 days. Then rats in HI and B groups were subjected to 30% TBSA full-thickness burn. Blood sample as well as heart, liver, lung,kidney, and stomach tissue samples were harvested from rats in B group at post burn hour(PBH) 2, 6, 12,24, 48, and 96 respectively for determination of serum content of H2S and CSE activity. Serum content of alanine transaminase (ALT) , aspartate aminotransferase(AST), MB isoenzyme of creatine kinase(CK-MB),urea nitrogen(BUN), and creatinine (Cr) in HI and B groups were examined at each time point. Samples were harvested from above organs in each group for histomorphological observation. Above-mentioned indexes were also determined in NC group as control. Data were processed with SNK-q test, t test,correlation analysis (between serum content of H2S and CSE activities, biochemical indexes). Results Serum content of H2S and CSE activities of above organs (except for lung tissue at PBH 48, 96) in B group within PBH 96 were lower than those in NC group, reaching minimum values at PBH 6 or 12. Compared with those in NC group, serum contents of all biochemical indexes in B group were obviously increased within PBH 48, in which serum contents of BUN [( 32.5 ± 9.8) mmoL/L] and Cr [( 65±9) μmol/L] reached peak at PBH 6, and serum contents of ALT [(423 ±59) U/L], AST [(993 ±60) U/L], and CK-MB [(49 261 ±6637) U/L] peaked at PBH 12. Serum contents of all biochemical indexes in HI group at each time point were significantly decreased as compared with those in B group, but the same change tendencies were showed in both groups. Histomorphological observation showed that all the organs were severely injured in B group at PBH 24, whereas those in HI group were markedly ameliorated. Serum content of H2S in B group was respectively correlated with CSE activities of all organs(with r value from 0. 639 to 0. 894, P values all below 0. 005) and serum contents of biochemical indexes(with r value from 0. 301 to 0. 585, P values all below 0.001). Conclusions H2S/CSE system may take part in pathophysiological process in rats with severe burn. Exogenous H2S replacement therapy can protect important organs of rats with severe burn.
