CAJ | 학술논문

目的观察戊二醛对静脉移植物内膜增生的影响.方法 60只新西兰大白兔随机分为实验组和对照组,实验组静脉移植物用1%戊二醛溶液浸泡10 min,对照组用生理盐水浸泡10 min.分别于移植后4、12周时观察内膜增生情况;对平滑肌细胞肌动蛋白α(α-actin)、增殖细胞核抗原(PCNA)行免疫组织化学染色;行原位末端标记法(TUNEL)染色观察细胞凋亡.结果术后4周和12周,实验组平滑肌细胞明显少于对照组,内膜厚度(0.016±0.004)、(0.031±0.008)mm小于对照组(0.025±0.005)、(0.093±0.024)m(P<0.05),但两组细胞增殖率差异无统计学意义(P>0.05).细胞凋亡率实验组(3.3±0.7)%、(6.7±1.5)%高于对照组(1.2±0.4)%、(1.8±0.5)%(P<0.05).结论戊二醛处理静脉移植物可显著减少移植物平滑肌细胞数目并抑制内膜增生.
목적 관찰무이철대정맥이식물내막증생적영향.방법 60지신서란대백토수궤분위실험조화대조조,실험조정맥이식물용1%무이철용액침포10 min,대조조용생리염수침포10 min.분별우이식후4、12주시관찰내막증생정황;대평활기세포기동단백α(α-actin)、증식세포핵항원(PCNA)행면역조직화학염색;행원위말단표기법(TUNEL)염색관찰세포조망.결과 술후4주화12주,실험조평활기세포명현소우대조조,내막후도(0.016±0.004)、(0.031±0.008)mm소우대조조(0.025±0.005)、(0.093±0.024)m(P<0.05),단량조세포증식솔차이무통계학의의(P>0.05).세포조망솔실험조(3.3±0.7)%、(6.7±1.5)%고우대조조(1.2±0.4)%、(1.8±0.5)%(P<0.05).결론 무이철처리정맥이식물가현저감소이식물평활기세포수목병억제내막증생.
Objective To investigate the effect of treatment of jugular vein graft with glutaraldehyde on intimal hyperplasia in rabbits. Methods Sixty New Zealand white rabbits were randomly divided into experimental group and the control group. Before the anastomosis, the vein in the experimental group was soaked in 1% glutaraldehyde solution for 10 min, and that in control group in normal saline for 10 min. The vein graft was harvested after 4 and 12 weeks. HE staining, smooth muscle cell α-actin and proliferating cell nuclear antigen ( PCNA ) immunohistochemical staining were performed. Apoptosis was detected by TUNEL. Results Four and 12 weeks after grafting, the number of smooth muscle cells in the experimental group was significantly less than that in the control group, and intima thickness [(0. 016 ±0. 004), (0. 031 ±0. 008) mm]in the experimental group was thinner than in the control group [(0. 025± 0. 005), (0. 093 ± 0. 024 ) mm, ( P < 0. 05 )]. However, there was no statistically significant difference in the rate of cell proliferation between two groups ( P > 0. 05 ). The apoptosis rate in the experimental group was higher than in the control group [(3. 3 ± 0. 7 ) %, (6. 7 ± 1. 5) % vs ( 1.2 ± 0. 4) %, ( 1.8 ±0. 5 ) %]in 4 and 12 weeks after grafting ( P < 0. 05 ). Conclusion Treatment of vein graft with glutaraldehyde can significantly reduce the number of graft smooth muscle cells and inhibit intimal hyperplasia.