CAJ | 학술논문

目的建立EB病毒感染和鼻咽癌血清学检测的敏感,特异和有效的方法.方法以EB病毒早期蛋白胸腺嘧啶激酶为检测抗原,建立ELISA和免疫纤维膜条方法,检测血清中的特异性的IgG抗体.结果用ELISA和诊断条同时检测了401份血清,其中鼻咽癌患者血清127份.两种方法检测鼻咽癌患者血清抗体均阳性,274份门诊患者血清中,55份ELISA和诊断条检测抗体均阳性,3份诊断条方法检测阳性的血清,ELISA检测A值接近临界值.对胸腺嘧啶激酶的抗体阳性率明显地高于早期蛋白P54.结论以胸腺嘧啶激酶为检测抗原,为鼻咽癌血清学诊断和高危人群的筛查提供更有效的手段.
목적 건립EB병독감염화비인암혈청학검측적민감,특이화유효적방법.방법 이EB병독조기단백흉선밀정격매위검측항원,건립ELISA화면역섬유막조방법,검측혈청중적특이성적IgG항체.결과 용ELISA화진단조동시검측료401빈혈청,기중비인암환자혈청127빈.량충방법검측비인암환자혈청항체균양성,274빈문진환자혈청중,55빈ELISA화진단조검측항체균양성,3빈진단조방법검측양성적혈청,ELISA검측A치접근림계치.대흉선밀정격매적항체양성솔명현지고우조기단백P54.결론 이흉선밀정격매위검측항원,위비인암혈청학진단화고위인군적사사제공경유효적수단.
Objective Development of new methods, ELISA and immunostrip test, for the diagnosis of nasopharyngeal carcinoma. Methods The engineering purified antigens coat plate or absorb on nitrocellulose filter. The plate and diagnostic strips carrying antigens were used for detection of IgG antibody in the sera from nasopharyngeal carcinoma patients and outpatients patients. Results 127 cases sera from nasopharyngeal carcinoma patients were parallel detected TK/ IgG antibody by ELISA and immunostrips. The TK/IgG antibody are all positive in the 127 cases of nasopharyngeal carcinoma patients. 55 cases show positive by ELISA, 58 cases positive by immunostrips in 247 cases sera from outpatient. The antibody positive rate to early antigen p54 lower then to TK. Conclusion ELISA and imuunostrips are sensitive and specific means for detection of the IgG antibody to TK of EBV and the diagnosis of nasopharyngeal carcinoma.