中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2012年
4期
307-310
,共4页
满孝蕊%胡绍燕%吴水燕%岑建农%陈子兴
滿孝蕊%鬍紹燕%吳水燕%岑建農%陳子興
만효예%호소연%오수연%잠건농%진자흥
基因,IGFBP7%细胞系,U937%RNA干扰
基因,IGFBP7%細胞繫,U937%RNA榦擾
기인,IGFBP7%세포계,U937%RNA간우
Gene,insulin-like growth factor binding protein 7 (IGFBP7)%Cells line,U937%RNA interference
目的 探讨胰岛素样生长因子结合蛋白7(IGFBP7)基因表达下调对急性髓系白血病细胞系U937细胞增殖及侵袭能力的影响.方法 利用肝癌细胞株SMMC7721细胞,筛选最有效的IGFBP7干扰序列,瞬时转染处于对数生长期U937细胞,用Western blot法检测转染后U937细胞IGFBP7蛋白表达.观察转染IGFBP7基因干扰序列后U937细胞的增殖、黏附、穿膜和浸润能力.结果 IGFBP7基因干扰序列转染24 h后U937细胞增殖能力明显下降,明显低于对照1组(未转染siRNA组)和对照2组(阴性干扰序列组)(A值分别为0.580 ±0.159、1.049 ±0.274、0.946±0.195)(P<0.01).IGFBP7基因下调后的U937细胞黏附于人脐静脉内皮细胞系ECV304细胞的数量明显低于对照1组和对照2组(A值分别为0.247±0.031对0.406±0.023和0.395±0.011)(P<0.01);穿越ECV304细胞包裹的细胞培养小室到底层的细胞明显减少[(0.387±0.021)×105对(1.017±0.031)×105、(0.908±0.027)×105];实验组黏附于Matrigel基质膜的细胞数明显低于对照组[(0.197±0.098)×105对(0.493±0.067)×105和(0.469±0.083)×105],差异有统计学意义(P值均<0.01).结论 IGFBP7基因通过影响U937细胞增殖以及与基质内皮细胞的黏附、穿膜、侵袭能力而参与急性髓系白血病的发生、发展过程.
目的 探討胰島素樣生長因子結閤蛋白7(IGFBP7)基因錶達下調對急性髓繫白血病細胞繫U937細胞增殖及侵襲能力的影響.方法 利用肝癌細胞株SMMC7721細胞,篩選最有效的IGFBP7榦擾序列,瞬時轉染處于對數生長期U937細胞,用Western blot法檢測轉染後U937細胞IGFBP7蛋白錶達.觀察轉染IGFBP7基因榦擾序列後U937細胞的增殖、黏附、穿膜和浸潤能力.結果 IGFBP7基因榦擾序列轉染24 h後U937細胞增殖能力明顯下降,明顯低于對照1組(未轉染siRNA組)和對照2組(陰性榦擾序列組)(A值分彆為0.580 ±0.159、1.049 ±0.274、0.946±0.195)(P<0.01).IGFBP7基因下調後的U937細胞黏附于人臍靜脈內皮細胞繫ECV304細胞的數量明顯低于對照1組和對照2組(A值分彆為0.247±0.031對0.406±0.023和0.395±0.011)(P<0.01);穿越ECV304細胞包裹的細胞培養小室到底層的細胞明顯減少[(0.387±0.021)×105對(1.017±0.031)×105、(0.908±0.027)×105];實驗組黏附于Matrigel基質膜的細胞數明顯低于對照組[(0.197±0.098)×105對(0.493±0.067)×105和(0.469±0.083)×105],差異有統計學意義(P值均<0.01).結論 IGFBP7基因通過影響U937細胞增殖以及與基質內皮細胞的黏附、穿膜、侵襲能力而參與急性髓繫白血病的髮生、髮展過程.
목적 탐토이도소양생장인자결합단백7(IGFBP7)기인표체하조대급성수계백혈병세포계U937세포증식급침습능력적영향.방법 이용간암세포주SMMC7721세포,사선최유효적IGFBP7간우서렬,순시전염처우대수생장기U937세포,용Western blot법검측전염후U937세포IGFBP7단백표체.관찰전염IGFBP7기인간우서렬후U937세포적증식、점부、천막화침윤능력.결과 IGFBP7기인간우서렬전염24 h후U937세포증식능력명현하강,명현저우대조1조(미전염siRNA조)화대조2조(음성간우서렬조)(A치분별위0.580 ±0.159、1.049 ±0.274、0.946±0.195)(P<0.01).IGFBP7기인하조후적U937세포점부우인제정맥내피세포계ECV304세포적수량명현저우대조1조화대조2조(A치분별위0.247±0.031대0.406±0.023화0.395±0.011)(P<0.01);천월ECV304세포포과적세포배양소실도저층적세포명현감소[(0.387±0.021)×105대(1.017±0.031)×105、(0.908±0.027)×105];실험조점부우Matrigel기질막적세포수명현저우대조조[(0.197±0.098)×105대(0.493±0.067)×105화(0.469±0.083)×105],차이유통계학의의(P치균<0.01).결론 IGFBP7기인통과영향U937세포증식이급여기질내피세포적점부、천막、침습능력이삼여급성수계백혈병적발생、발전과정.
Objective To explore the effect of down-regulation of insulin-like growth factor binding protein 7 (IGFBP7 ) on the proliferation and invasiveness of leukemia cell line U937 cells. Methods Three pairs of double-strand siRNA targeting IGFBP7 gene were transfected into SMMC7721 cells to select the most efficient one for U937 cells. qRT-PCR and Western blot were used to detect the expression of IGFBP7 in U937 cells after transiently transfected with siRNA of IGFBP7. Cell proliferation, adhesion, trans-endothelial migration and invasion were performed in transfected cells and control groups. Results After transfected with siRNA of IGFBP7 in U937 cells, the ability of cell proliferation was significantly decreased at 24 h (0. 580 ± 0. 159 ) compared to that of parental cells and scramble negative control( 1. 049 ± 0. 274, 0. 946 ± 0. 195 , respectively) (P < 0. 01 ). Adhesion of U937 cells transfected with IGFBP7 gene specific siRNA to ECV304 cells was significantly lower than that of the control groups (0. 247 ± 0. 031 vs 0. 406 ± 0. 023 and 0. 395 ± 0.011)(P<0.01). Transendothelial membrane of U937 cells into the bottom of the 24-well plate for experimental group were less than those in the control groups [(0. 387 ±0. 021) × 105 vs ( 1. 017 ±0. 031 )× 105 and (0.908 ±0.027) × 10]. Cells adherent to the matrigel for experimental group were less than those in the control groups [(0. 197 ± 0.098)× 105 vs (0. 493 ± 0. 067) × 105 and (0. 469 ± 0. 083) × 105]. The difference was significant (P < 0. 01) . Conclusion IGFBP7 gene plays a contributing role in leukemogene-sis involving in leukemic cells' proliferation and interaction with endothelial cells through adhesion, invasion and migration.
