CAJ | 학술논문

目的探讨Survivin和Caspase-3在子宫平滑肌肿瘤中的表达及其意义,在富于细胞型平滑肌瘤和子宫平滑肌肉瘤的鉴别诊断中的意义.方法采用免疫组织化学二步法分别检测30例富于细胞型子宫平滑肌瘤细胞、10例正常子宫平滑肌细胞、10例普通型子宫平滑肌瘤细胞、15例子宫平滑肌肉瘤细胞中Survivin和Caspase-3的表达.结果 Survivin在正常子宫肌层组织中仪有个别弱表达,在子宫普通型平滑肌瘤、富于细胞型平滑肌瘤、平滑肌肉瘤中的表达呈上升趋势,且普通型组和肉瘤组差别有统计学意义(P<0.05);而Caspase-3在正常子宫肌层、普通型平滑肌瘤、富于细胞型平滑肌瘤、平滑肌肉瘤中的表达呈下降趋势.且正常组与富于细胞型组、正常组与肉瘤组之间差异有统计学意义(P<0.05).结论 Survivin可能通过抑制Caspase-3的活性来抑制细胞凋亡,延长细胞寿命,从而在子宫平滑肌肿瘤由良性发展为恶性的过程中发挥重要作用.二者联合检测有助于子宫平滑肌肿瘤之间的鉴别诊断.
목적 탐토Survivin화Caspase-3재자궁평활기종류중적표체급기의의,재부우세포형평활기류화자궁평활기육류적감별진단중적의의.방법 채용면역조직화학이보법분별검측30례부우세포형자궁평활기류세포、10례정상자궁평활기세포、10례보통형자궁평활기류세포、15례자궁평활기육류세포중Survivin화Caspase-3적표체.결과 Survivin재정상자궁기층조직중의유개별약표체,재자궁보통형평활기류、부우세포형평활기류、평활기육류중적표체정상승추세,차보통형조화육류조차별유통계학의의(P<0.05);이Caspase-3재정상자궁기층、보통형평활기류、부우세포형평활기류、평활기육류중적표체정하강추세.차정상조여부우세포형조、정상조여육류조지간차이유통계학의의(P<0.05).결론 Survivin가능통과억제Caspase-3적활성래억제세포조망,연장세포수명,종이재자궁평활기종류유량성발전위악성적과정중발휘중요작용.이자연합검측유조우자궁평활기종류지간적감별진단.
Objective To study the expression of Survivin and Caspase-3 in uterine smooth muscle tumour (USMT) and it' s significance. Methods Expression of Survivin and Caspase-3 protein were determined by immunohistochemistry Two-step method on 30 uterine Cellular Uterine Leiomyoma (CUL), 10 normal uterine smooth muscle (NSM), 10 Ordinary Uterine Leiomyoma (OUL), 15 Leiomysarcoma (LMS). Results Survivin level in normal uterine smooth muscle are very low, and in OUL, CUL, LMS was on increasing trend, the OL group and the LCA group have statistical significant difference(P<0.05). Caspase-3 level in NSM, OUL, CUL, LMS was on decreasing trend. The NSM group and the CL group, the NSM group and the LCA group both have statistical significant difference (P<0.05). Conclusion Maybe Survivin can inhibit apoptosis and extend cells lives by inhibit the activity of Caspase-3, so it played an important role in the development progress from benign uterine tumour to malignant uterine tumour. The detect of Survivin and Caspase-3 may be useful in the differential diagnosis of uterine smooth muscle tumours.